Fig 2: STLC resistance of both U-R and H-R cells is dependent on KIF15/kinesin-12.(A) Experimental procedure. (B) Evaluation of knockdown efficiency by immunoblotting. (C) The effect of KIF15 depletion on STLC resistance of U-R and H-R cells was examined by siRNA-mediated gene knockdown. Relative cell viability for a given STLC concentration is shown as mean ± SD (n = 3). KIF15 depletion substantially reduced the STLC resistance of U-R and H-R cells.

Fig 3: Effect of KIF11 or KIF15 depletion on growth of U-R or H-R cells.(A) Experimental procedure. (B) Evaluation of knockdown efficiency by immunoblotting. (C) KIF11 knockdown heavily compromised the growth of the U-R cells, while the growth of H-R cells was affected only when both KIF11 and KIF15 were depleted, suggesting their mutually compensatory relationship. Cell viability was shown relative to that for the cells treated with control siRNA.