PIWI/HIWI Antibody orb19551 from Biorbyt

Product Specs
ItemPIWI/HIWI Antibody
CompanyBiorbyt
Price $530.00Supplier Page
Catalog Numberorb19551
Quantity100 ug
Conjugate/TagUnconjugated
ReactivityHuman
ApplicationsELISA, FC, IF, IHC
TargetPIWI / HIWI
HostGoat
Molecular Weight98.6; 94.8
ClonalityPolyclonal
NCBI Full Gene Namepiwi like RNA-mediated gene silencing 1
NCBI Gene AliasesCT80.1, HIWI, MIWI, PIWI
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Research AreaDevelopmental Biology, Epigenetics

Specifications/Features

Application Notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments showed a 100kDa band but also bands at approx 35kDa and 26-28kDa in Human Ovary, Placenta, Prostate and Testis lysates after 0.1 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional bands we observe given the calculated size of 98.6kDa according to NP_004755.2. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Application Dilutions: ELISA: 1:64000, WB: 0.1 µg/ml, IHC-P: 4-6 µg/ml