SPDEF Rabbit Polyclonal Antibody orb13642 from Biorbyt

Product Specs
ItemSPDEF Rabbit Polyclonal Antibody
CompanyBiorbyt
Price $250.00Supplier Page
Catalog Numberorb13642
Quantity50 ul, 100 ul, 200 ul
Conjugate/TagUnconjugated
ReactivityHuman, Mouse, Rat
TargetSPDEF
ApplicationsICC, IF, IHC-Fr, IHC-P
ClonalityPolyclonal
FormatLiquid
ImmunogenKLH conjugated synthetic peptide derived from human PDEF (251-335/335aa)
Molecular Weight38 kDa
IsotypeIgG
HostRabbit
Concentration1mg/ml
NCBI Full Gene NameSAM pointed domain containing ETS transcription factor
NCBI Gene AliasesPDEF, bA375E1.3
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Research AreaCancer Research, DNA and RNA, DNA and RNA Research, Transcription Factors, Transcription Regulators, Tumor Biomarkers

Specifications/Features

Application Dilutions: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500

Questions and Answers

Can this antibody be used to study the role of SPDEF in neuroendocrine prostate cancer?

Yes, the PDEF antibody has been utilized in research related to neuroendocrine prostate cancer, as indicated in the study by Beltran et al. (2016). In their experiments, the antibody was employed for Western blot analysis to assess SPDEF expression in cell lines treated with enzalutamide, highlighting its relevance in understanding treatment resistance mechanisms in prostate cancer.

What species can I use this antibody on for my experiments?

The PDEF antibody is reactive with mouse, human, and rat samples, making it suitable for a variety of experimental models across these species.

What applications is this antibody suitable for in my research?

The PDEF antibody is shown to work in several applications, including ELISA, immunocytochemistry, immunofluorescence, and both frozen and paraffin-embedded tissue immunohistochemistry. This versatility allows for its use in various experimental setups.

What is the recommended dilution for using this antibody in immunohistochemistry?

For immunohistochemistry on paraffin-embedded tissues, the recommended dilution for the PDEF antibody is between 1:100 and 1:500, which can be optimized based on specific experimental conditions.