SLAMF9 antibody

Blank control: (mo)splenocytes(blue) Isotype Control Antibody: Rabbit IgG-FITC(orange) ; Primary Antibody Dilution: 1μl in 100 μL1X PBS containing 0.5% BSA(green).|Sample: A549 (human)cell Lysate at 40 ug BV2 (mouse)cell Lysate at 40 ug Primary: Anti- SLAMF9 at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30kD Observed band size: 35 kD|Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with PBST for 30 min on room temperature) Primary Antibody (rad line): Rabbit Anti-SLAMF9 antibody; Dilution: 1μg /10^6 cells; Isotype