Phospho-mTOR (Ser2448) (49F9) Rabbit mAb 2976S from Cell Signaling Technology

Product Specs
ItemPhospho-mTOR (Ser2448) (49F9) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number2976S
Quantity100 µl
ApplicationsIHC-P
ReactivityH
TargetmTOR (Ser2448) phosphate
ModificationsmTOR (Ser2448) phosphate
HostRabbit
NCBI Gene AliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
ClonalityMonoclonal
NCBI Full Gene Namemechanistic target of rapamycin kinase
Research AreaPI3K / Akt Signaling
IsotypeIgG
Clone49F9
Molecular Weight289

Questions and Answers

Can this antibody be used to study mTOR activity in human tissues?

Yes, the Phospho-mTOR (Ser2448) Rabbit mAb has been utilized in immunohistochemistry to detect phosphorylated mTOR in various human tissues, as evidenced by its application in studies examining mTOR signaling pathways in conditions such as glioblastoma and prostate cancer.

Is there any evidence of this antibody being used in research related to cancer?

The antibody has been cited in multiple studies investigating the role of mTOR in cancer. For instance, it was used in research demonstrating that mTOR activity is linked to the metabolic adaptations of glioma cells and the response to therapeutic agents, highlighting its relevance in cancer biology.

How does this antibody perform in detecting mTOR phosphorylation in experimental models?

The Phospho-mTOR (Ser2448) Rabbit mAb has shown effectiveness in detecting phosphorylated mTOR in various experimental models, including primary neuronal cultures and 3D bioprinted cancer models, indicating its utility in diverse research contexts.

What specific applications have been documented for this antibody in scientific literature?

This antibody has been documented for use in immunohistochemistry, particularly in studies assessing the phosphorylation status of mTOR in response to various stimuli, such as IL-6 in prostate cancer cells and in models of neurodegeneration, demonstrating its versatility in different research areas.

Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Cellular interactions in the sentinel lymph node predict melanoma recurrencebioRxivDecember 17, 2025Sabrina M. Solis, Yang Yang, Yee Hoon Foong, Anushka Dheer, Kevin L. Ma, Mohammad Saad Farooq, Shira G. Rosenberg, Sophie L. Gray-Gaillard, Giorgos C. Karakousis, Xiaowei Xu, et al.89356BF Anti-human CXCR5 (EPR23463-30),Abcam,Cat# ab272936 Anti-human PMTOR (49F9),Cell Signaling Technology,Cat# 2976S Anti-human BCA1 (EPR23400-92),Abcam,Cat# ab246518 Anti-human IL-21R (Polyclonal),Leinco Technologies,Cat#

(2) UBE2S inhibits colorectal cancer proliferation by regulating the PI3K/AKT and MAPK/ERK pathways via TRAF6Sci RepOctober 13, 2025Jun Li, Haoran Feng, Kun Liu, Haiyan Huang, Xiaopin Ji, Tao Zhang, Xi Cheng, Ren ZhaoPhospho-AKT(Ser473, 1:1000, 4060, CST), mTOR(1:1000, 2983, CST), Phospho-mTOR(Ser2448, p-mTORC1, 1:1000, 5536 and 2976, CST), Phospho-mTOR(Ser2481, p-mTORC2, 1:1000, 2974, CST), Phospho-AP-1(p-c-Jun, 1:1000, 9261, CST),

(3) A study of oral metformin for the intravesical treatment of non‐muscle‐invasive bladder cancerBJU InternationalOctober 1, 2025Jons W. van Hattum, Marinka J. Remmelink, Sieb T. Nuijens, Ben Max de Ruiter, Gerrit K.J. Hooijer, Jorg R. Oddens, C. Dilara Savci‐Heijink, Ron Mathot, J. Alfred Witjes, Michael .N. Pollak, et al.phosphorylated‐AMPK (Cell Signalling, #2535), mTOR (Cell Signalling, #2983), phosphorylated‐MTOR (Cell Signalling, #2976), p70S6 kinase (Cell Signalling, #34475), phosphorylated‐p70S6 kinase (Santa Cruz Biotechnology, #8416)

(4) Molecularly matched targeted therapies plus radiotherapy in glioblastoma: the phase 1/2a N2M2 umbrella trialNature MedicineSeptember 5, 2025Wolfgang Wick, Lisa-Marie Lanz, Antje Wick, Inga Harting, Susan Dettmer, Abigail K. Suwala, Ralf Ketter, Ghazaleh Tabatabai, Corinna Seliger, Martin Glas, et al.using a heat antigen retrieval procedure (citrate buffer) and the phospho-mTOR antibody (Ser-2448; 2976, Cell Signaling Technology) in a dilution of 1:100 according to the manufacturer’s recommendations. DNA methylation

(5) Efficacy of ATR Kinase Inhibitor Elimusertib Monotherapy or Combination in Tumors with DNA Damage Response Pathway and Other Genomic AlterationsMolecular Cancer TherapeuticsSeptember 2, 2025Kaushik Varadarajan, Christian X. Cruz Pico, Kurt W. Evans, Maria Gabriela Raso, Yasmeen Qamar Rizvi, Xiaofeng Zheng, Dhruv Chachad, Timothy P. DiPeri, Bailiang Wang, Stephen M. Scott, et al.Cell Signaling Technology, RRID: AB_331682) with a dilution of 1:100, phospho-mTOR (Ser2448; 49F9) rabbit mAb (catalog #2976, Cell Signaling Technology, RRID: AB_490932) with a dilution of 1:100, Ki67 mouse mAb, (catalog #M7240,

(6) Natural product Erianin: mitigating FOLFOX toxicity and enhancing against colorectal cancerFrontiers in ChemistryAugust 22, 2025Fuqin Yang, Zeqiao Li, Haishan Zhang, Mingxiao Zhang, Zhenwei He, Xiaoqin Zheng, Yingru Zhu, Pei Long, Ruirui Ding, Zhengbin Lin, et al.AKT1 (CST, #75692, 1:400), p-AKT1 (abcam, #ab81283, 1:400), mTOR (CST, #2983, 1:400), p-mTOR (CST, #2976, 1:400). The sections were incubated with HRP-labeled secondary antibody (Servicebio, GB23303, GB23301,

(7) Prenatal valproic acid on the basis of gestational diabetes also induces autistic behavior and disrupts myelination and oligodendroglial maturation slightly in offspringTransl PsychiatryAugust 7, 2025Maolin Li, Zhifei Qiao, Jizheng Li, Hongli Zhou, Dong Huang, Yan Cai, Xiaolong Li, Zuo Zhang, Jianyun Zhou, Jiyin ZhouT,"Immunoblotting, (1:1000)",USA 20,Antibody,Phospho-mTOR (Ser2448) (49F9) Rabbit mAb,Cell Signaling Technology,2976S,"Immunoblotting, (1:1000)",USA 21,Antibody,Akt (pan) (11E7) Rabbit mAb,Cell Signaling Technology,4685S,"Immunoblotting,

(8) Long Non‐Coding RNA IGFRIL Couples with PTBP1 to Destabilize IGFBP3 mRNA to Promote the IGF1R‐AKT‐mTOR Axis and Hepatocellular CarcinomaAdvanced ScienceJuly 21, 2025Jing Zhang, Chengming Gao, Haibei Li, Yuying Chen, Liting Yang, Qian Jin, Lan Feng, Xia Wang, Xinyi Liu, Hongxia Chen, et al.Proteintech, USA), anti‐p‐AKT (1:500; 4060, CST, USA), anti‐mTOR (1:500; 2972, CST, USA), anti‐p‐mTOR (1:500; 2976, CST, USA), anti‐S6K (1:500; 2708, CST, USA), anti‐p‐S6K (1:500; 9234, CST, USA), anti‐4EBP1 (1:500;

(9) Antitumor Effect of mTOR1/2 Dual Inhibitor AZD8055 in Canine Pulmonary CarcinomaCancers (Basel)June 14, 2025Tomokazu Nagashima, Kazuhiko Ochiai, Yuka Takizawa, Youta Koike, Takahiro Saito, Asumi Muramatsu, Daigo Azakami, Yukino Machida, Makoto Bonkobara, Toshiyuki Ishiwata, et al.1:1000; cat. no. 4060, CST), mTOR (1:1000; cat. no. 2983, CST), phospho-mTOR (Ser2448; 1:1000; cat. no. 2976, CST), 4E-BP (1:1000; cat. no. 9644, CST) and phospho-4E-BP (Thr37/46; 1:1000; cat. no. 2855, CST),

(10) The anti-diabetic PPARγ agonist Pioglitazone inhibits cell proliferation and induces metabolic reprogramming in prostate cancerMol CancerMay 5, 2025Emine Atas, Kerstin Berchtold, Michaela Schlederer, Sophie Prodinger, Felix Sternberg, Perla Pucci, Christopher Steel, Jamie D. Matthews, Emily R. James, Cécile Philippe, et al.phospho AMPKα (Thr 172),Cell Signaling,#2535 Rabbit monoclonal phospho mTOR (Ser2448),Cell Signaling,#2976 "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(11) Temperature regulated nutrient sensing and metabolism of amino acids in juvenile turbot (Scophthalmus maximus L.)Marine Life Science & TechnologyApril 2, 2025Xuemin Zhang, Jiru Wang, Chengdong Liu, Xuan Wang, Huihui Zhou, Kangsen Mai, Gen He,Cell signaling technology,4856 mTOR,Cell signaling technology,2972 phospho-mTOR (Ser2448),Cell signaling technology,2976 eIF2α,Cell signaling technology,9722 phosphor-eIF2α,Cell signaling technology,3579 Bip (Grp78),Cell signaling technology

(12) Cachexia Alters Central Nervous System Morphology and Functionality in Cancer PatientsJ Cachexia Sarcopenia MuscleFebruary 17, 2025Estefania Simoes, Ricardo Uchida, Mariana P. Nucci, Fabio L. S. Duran, Joanna D. C. C. Lima, Leonardo R. Gama, Naomi A. Costa, Maria C. G. Otaduy, Fang C. Bin, Jose P. Otoch, et al.assessed: TSC1 (Abcam ab40872), TSC2 (Abcam ab32554), mTOR (Cell signalling 2983), pmTOR (Cell signalling 2976), p70S6K (Cell signalling 2708) and p‐p70S6K (Invitrogen PA5‐38307markers). Detailed IHC protocol is

(13) Spermine Synthase : A Potential Prognostic Marker for Lower-Grade GliomasJournal of Korean Neurosurgical SocietyJanuary 1, 2025Chen Liu, Hongqi Li, Xiaolong Hu, Maohui Yan, Zhiguang Fu, Hengheng Zhang, Yingjie Wang, Nan Du(4695; CST), anti-p-ERK antibody (4376; CST), anti-mTOR antibody (2983; CST), anti-p-mTOR antibody (2976; CST), anti-E-cadherin antibody (3195; CST), anti-snail antibody (3895; CST), and anti-Vimentin antibody

(14) RNF19A inhibits bladder cancer progression by regulating ILK ubiquitination and inactivating the AKT/mTOR signalling pathwayBiology DirectNovember 6, 2024Hao Deng, Guanghai Ji, Jun Ma, Jun Cai, Shaoping Cheng, Fan ChengCell Signaling Technology), anti-p-AKT (1:200; Cat: #4060; Cell Signaling Technology), and anti-p-mTOR (1:200; Cat: #2976; Cell Signaling Technology). Statistical analysis All the data were statistically analysed using GraphPad

(15) JAML overexpressed in colorectal cancer promotes tumour proliferation by activating the PI3K-AKT-mTOR signalling pathwayScientific ReportsOctober 18, 2024Yuying Fang, Yanan Liu, Zhilin Dong, Xinchao Zhao, Mingyan Zhang, Yawen Zheng, Chunsheng Yang, Yufeng Wang, Ning Liu, Peng Yan, et al.Cell Signaling Technology, Cat No. 3285), anti-phospho-mTOR (1:1000, Cell Signaling Technology, Cat No. 2976), anti-mTOR (1:1000, Cell Signaling Technology, Cat No. 2983), anti-GAPDH (1:20,000, Proteintech, Cat

(16) Proteogenomic characterization of pancreatic neuroendocrine tumors uncovers hypoxia and immune signatures in clinically aggressive subtypesiScienceAugust 16, 2024Atsushi Tanaka, Makiko Ogawa, Yihua Zhou, Yusuke Otani, Ronald C. Hendrickson, Matthew M. Miele, Zhuoning Li, David S. Klimstra, Julia Y. Wang, Michael H. Roehrl1:50),Abcam,Cat. # ab81283; RRID: AB_2224551 Rabbit monoclonal anti-p-mTOR (dilution 1:200),Cell Signaling,Cat. # 2976; RRID: AB_490932 Rabbit monoclonal anti-IRF1 (dilution 1:100),Abcam,Cat. # ab243895; RRID: AB_2832955 Rabbit

(17) Poorly cohesive gastric cancer with increased epithelial‑mesenchymal transition is associated with a poor prognosisOncology LettersJuly 3, 2024Nobuhiro Nakazawa, Makoto Sohda, Munenori Ide, Yuki Shimoda, Akihiko Sano, Makoto Sakai, Tetsunari Oyama, Ken Shirabe, Hiroshi SaekiProteintech Group, Inc.), p-mTOR antibodies (1:80 dilution; anti-rabbit monoclonal antibody; cat. no. #2976; Cell Signaling Technology, Inc.) and E-cadherin antibodies (1:500 dilution; HECD-1; mouse monoclonal;

(18) GIP attenuates neuronal oxidative stress by regulating glucose uptake in spinal cord injury of ratCNS Neuroscience & TherapeuticsJune 17, 2024Beibei Guo, Mengwei Qi, Xiaoqian Luo, Longyu Guo, Man Xu, Yufang Zhang, Zhen Li, Mingxuan Li, Ronghua Wu, Tuchen Guan, et al.1:1000, Cat. 5661, CST; rabbit anti‐mTOR, 1:1000, Cat. 2983, CST; rabbit anti‐P‐mTOR, 1:1000, Cat. 2976, CST; rabbit anti‐BAX, 1:1000, Cat. 2772 T, CST; rabbit anti‐Bcl2, 1:1000, Cat. 26,593‐1‐AP, Proteintech;

(19) Tumorigenic role of tacrolimus through mTORC1/C2 activation in post-transplant renal cell carcinomasBritish Journal of CancerApril 20, 2024Dorottya Moldvai, Dániel Sztankovics, Titanilla Dankó, Enikő Vetlényi, Gábor Petővári, Ágnes Márk, Attila Patonai, Gyula Végső, László Piros, Ádám Hosszú, et al.Slides were incubated with primary antibodies: anti-p-(Ser2448)-mTOR (Cell Signaling; USA (MA); cat#2976; 1:100), anti-p-(Ser235/236)-S6 (Cell Signaling; cat#2211; 1:100), anti-Rictor (Bethyl; USA (TX); cat#A500-002A;

(20) Proteogenomic characterization of primary colorectal cancer and metastatic progression identifies proteome-based subtypes and signaturesCell ReportsFebruary 19, 2024Atsushi Tanaka, Makiko Ogawa, Yihua Zhou, Kei Namba, Ronald C. Hendrickson, Matthew M. Miele, Zhuoning Li, David S. Klimstra, Patrick G. Buckley, Jeffrey Gulcher, et al.anti-pAKT,Abcam,Cat. # ab81283; RRID: AB_2224551 Rabbit monoclonal anti-p-mTOR,Cell Signaling,Cat. # 2976; RRID: AB_490932 Rabbit monoclonal anti-pERK,Cell Signaling,Cat. # 4370; RRID: AB_2315112 Mouse monoclonal

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Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific)

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-mTOR (Ser2448) (49F9) Rabbit mAb from Cell Signaling Technology

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