F-Actin Rabbit Polyclonal Antibody orb10068 from Biorbyt

Product Specs
ItemF-Actin Rabbit Polyclonal Antibody
CompanyBiorbyt
Price $250.00Supplier Page
Catalog Numberorb10068
Quantity50 ul, 100 ul, 200 ul
ApplicationsFC, IF, IHC-Fr, IHC-P, WB
Conjugate/TagUnconjugated
FormatLiquid
Concentration1mg/ml
ReactivityHuman, Mouse, Rat
TargetACTB
Molecular Weight42 kDa
IsotypeIgG
HostRabbit
ClonalityPolyclonal
ImmunogenSynthetic MAP peptide derived from human beta-Actin (1-50/374aa)
NCBI Full Gene Nameactin beta
NCBI Gene AliasesBRWS1, PS1TP5BP1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

Specifications/Features

Application Dilutions: WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3µg /test

Questions and Answers

Can this antibody be used to study actin levels in muscle tissues?

Yes, the F-Actin antibody has been utilized in studies examining actin levels in muscle tissues, as demonstrated in research investigating the ultrastructure of medial rectus muscles in patients with intermittent exotropia. In this study, the antibody was used in Western blotting to determine levels of actin, revealing significant differences in actin levels between adolescent and adult groups ().

What species can I use this antibody with for my experiments?

The F-Actin antibody is reactive with mouse, human (Homo sapiens), and rat species, making it suitable for a variety of biological studies across these organisms.

What applications is this antibody suitable for in my research?

This antibody has been shown to work in several applications, including Western blotting, ELISA, flow cytometry, and immunohistochemistry. Its versatility allows for a wide range of experimental setups in cell biology.

What dilution should I use for Western blotting with this antibody?

For Western blotting, the recommended dilution range for the F-Actin antibody is between 1:1000 and 1:5000, depending on the specific experimental conditions and the sensitivity required for detection.

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