Fig 1: Transcriptional profiling of mouse PPs after anti-a4ß7 therapya, UMAP representation of scRNA-seq analysis of cells isolated from PPs of mice, 24h after anti-a4ß7 antibody or PBS administration. b, Dotplot showing mean expression and proportion of expressing cells for the indicated genes in each cluster. c, UMAP plots showing expression level of the indicated genes. d, Bar plots indicating cell composition of PPs from anti-a4ß7- and PBS-treated mice. Cells were grouped into follicular B cells (FB), germinal center B cells (GC B), T cells (T) and Others. e, Bar plots for the frequency of FB cell clusters among total cells. Data shown as individual values and mean. Unpaired analysis was done using Mann-Whitney test, p value is indicated. f, Dotplot showing mean expression and proportion of expressing cells for the indicated genes in clusters 0, 2 and 8. g, (left) Bar plot of differentially expressed genes in cluster 8 cells between anti-a4ß7- and PBS-treated mice. (right) Expression level of Itgb7 in cells from cluster 12. h, Frequency of IgA+ plasma cells isolated from PPs. i, Mice were primed intraperitoneally with OVA + CT (day 0) and boosted orally with OVA + CT (days 7 and 14) and treated with anti-a4ß7 or isotype control antibody every 3 days. Feces were collected at day 21. j, OVA-specific IgA measured by ELISA. Data shown as individual values and mean. Unpaired analysis was done using Mann-Whitney test, p value is indicated.