Fig 1: Salvianolate mediates cardiomyocyte autophagy through miR-30a regulation of becn1 and reduced Dox/Azi-induced cardiotoxicity. (A) The real-time PCR analysis of miR-30a level in Dox treated Dox-induced cardiotoxicity zebrafish model. (B) Luciferase reporter gene assay in HEK 293T cells showed that miR-30a directly binds to the becn1 3′UTR. (C–F) miR-30a mimic or NC mimic treated Dox-induced cardiotoxicity zebrafish model pre-administrated with salvianolate or not. Heart edema rate at 3 dpf (C) heart rate at 3 dpf per min (D). Quantification of the density of autophagosomes in the myl7:EGFP-LC3 system (E). Quantification of the density of autophagosomes and autolysosomes in the myl7:mRFP-EGFP-LC3 system (F). All data are expressed as mean ± SEM, *P <0.05, †P <0.01, ‡P <0.001. DMSO: Dimethyl sulfoxide; dpf: Days post fertilization; Dox: Doxorubicin; EGFP: Efficiency green fluorescent protein; mRFP: Monomeric red fluorescent protein; NC: Normal control; PCR: Polymerase chain reaction; SEM: Standard error of mean; UTR: Untranslated region.