Immunofluorescence staining of RANGRF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with RANGRF Polyclonal Antibody (Product # PA5-118236, 1:100) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.

Western Blot using RANGRF Polyclonal Antibody (Product # PA5-118236) at 1:500 dilution. Lane A: HEK293 Whole Cell Lysate, Lane B: MOLT4 Whole Cell Lysate, Lane C: Daudi Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 24 kDa.