Western Blot analysis of PRDM9 in various cell lines using a PRDM9 Polyclonal antibody (Product # PA5-120725) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.

Western blot analysis of PRDM9 in extracts of various cell lines. Samples were incubated with PRDM9 Polyclonal antibody (Product # PA5-120725) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.