Mouse IgG1 Biotin Isotype Control MG115 from Thermo Fisher Scientific

Product Specs
ItemMouse IgG1 Biotin Isotype Control
CompanyThermo Fisher Scientific
Price Supplier Page
Catalog NumberMG115
Antibody TypeIsotype Control
FormatPBS with 0.1% sodium azide
ApplicationsFlow Cytometry
IsotypeIgG1
TargetMouse IgG1
Conjugate/TagBiotin
HostMouse
Quantity500 µL
Storage2-8°C
ClonalityMonoclonal

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

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Website: www.thermofisher.com

(1) E.coli-derived CsgA-peptides stimulate cytokine production in microglia and lead to transient changes in neocortical Aβ-levels in pre-plaque APPSWE/PS1ΔE9 and wild type miceBrain, Behavior, & Immunity - HealthDecember 1, 2025Leda Mygind, Gabriel Jensen, Victoria Neesgaard, Katrine Tækker Krohn, Sergi Torres Puig, Anders Boysen, Sussanne Petersen, Kate Lykke Lambertsen, Athanasios Metaxas, Michael Kemp, et al.in all stainings. Substitution controls with biotinylated mouse IgG1 isotype control (ThermoFisher, MG115) were devoid of Aβ-staining. 2.10 Quantitative real-time RT-PCR (Q-PCR) 2.10.1 Microglia RNA extraction

(2) HOS 15‐mediated turnover of PRR 7 enhances freezing toleranceNew PhytologistNovember 1, 2024Yeon Jeong Kim, Woe‐Yeon Kim, David E. Somersleupeptin, 1 μg/ml aprotinin, 1 μg/ml pepstatin, 5 μg/ml antipain, 5 μg/ml chymostatin, 50 μM MG132, 50 μM MG115, and 50 μM ALLN) from 1.5 ml of tissue powder. Protein G-agarose resin pretreated with mouse monoclonal

(3) Viperin-like proteins interfere with RNA viruses in plantsFrontiers in Plant ScienceJune 4, 2024Radwa Kamel, Rashid Aman, Magdy M. Mahfouzprotease inhibitors (PMSF, leupeptin, aprotinin, pepstatin, antipain, chymostatin, Na2VO3, NaF, MG132, and MG115). The protein extracts were separated on a 10% polyacrylamide gel. Immunoblot analysis was performed

(4) Novel heterozygous mutation in alpha-2-macroglobulin (A2M) suppressing the binding of amyloid-β (Aβ)Frontiers in NeurologyJanuary 9, 2023Guozhen Qiu, Liming Cao, Yong-Jun ChenPierce Protein A/G Magnetic Beads were incubated with HIS-tag antibody (ab18184, Abcam) for 4 h or IgG (MG115, Invitrogen) as a negative control and subsequently with the protein sample overnight at 4°C. Eluted

(5) Mild Microglial Responses in the Cortex and Perivascular Macrophage Infiltration in Subcortical White Matter in Dogs with Age-Related Dementia Modelling Prodromal Alzheimer’s DiseaseJournal of Alzheimer's DiseaseJuly 21, 2021Barbara Blicher Thomsen et al.respectively. Rabbit IgG (Dako X903), mouse IgG1 (Dako, X0931), and biotinylated mouse IgG1 (Invitrogen, MG115) were used for substitution control. Staining procedures for Aβ and Iba1 Sections were deparaffinized

(6) Mild Microglial Responses in the Cortex and Perivascular Macrophage Infiltration in Subcortical White Matter in Dogs with Age-Related Dementia Modelling Prodromal Alzheimer’s DiseaseJ Alzheimers DisMay 26, 2021Barbara Blicher Thomsen, Cecilie Madsen, Katrine Tækker Krohn, Camilla Thygesen, Trine Schütt, Athanasios Metaxas, Sultan Darvesh, Jørgen Steen Agerholm, Martin Wirenfeldt, Mette Berendt, et al.respectively. Rabbit IgG (Dako X903), mouse IgG1 (Dako, X0931), and biotinylated mouse IgG1 (Invitrogen, MG115) were used for substitution control. Staining procedures for Aβ and Iba1 Sections were deparaffinized

(7) Functional switching of NPR1 between chloroplast and nucleus for adaptive response to salt stressScientific ReportsDecember 1, 2020So Yeon Seo, Soo Jin Wi, Ky Young Park(1X phosphate-buffered saline, pH 7.4 (Cat. no. 10010–031, ThermoFisher Scientific, USA)) containing MG115, MG132, and plant protease inhibitor cocktail (Sigma, USA). Protein lysates (30 µg) were precleared

(8) MARCH5 mediates NOXA-dependent MCL1 degradation driven by kinase inhibitors and integrated stress response activationeLifeJune 2, 2020Arai S, Varkaris A, Nouri M, Chen S, Xie L, Balk SP.μM), gamitrinib-TPP (5 μM), CPI-613 (200 μM), or DMSO for 5 hr. (E) LNCaP cells were pretreated with MG115 (10 μM) and MG132 (10 μM) for 1 hr, followed by treatment with CPI-613 (200 μM), gamitrinib-TPP (5 μM)

(9) Pseudo Response Regulators Regulate Photoperiodic Hypocotyl Growth by Repressing PIF4/5 TranscriptionPlant PhysiologyJune 1, 2020Na Li, Yuanyuan Zhang, Yuqing He, Yan Wang, Lei WangmL21 pepstatin, 5 mg mL21 antipain, 5 mg mL21 chymostatin, 2 mM NaVO3, 2 mM NaF, 50 mM MG132, 50 mM MG115, and 50 mM ALLN). The cleared supernatant was incubated with Protein A beads (15918-014, Invitrogen)

(10) CRISPR-Cas13d mediates robust RNA virus interference in plantsGenome BiologyDecember 2, 2019Ahmed Mahas, Rashid Aman, Magdy Mahfouzprotease inhibitors (PMSF, leupeptin, aprotinin, pepstatin, antipain, chymostatin, Na2VO3, NaF, MG132, and MG115). Proteins were separated on a 10% polyacrylamide gel. Immunoblot analysis was conducted using mouse

(11) Hop2 Interacts with ATF4 to Promote Osteoblast DifferentiationJournal of Bone and Mineral ResearchDecember 1, 2019Yang Zhang, Tonghui Lin, Na Lian, Huan Tao, Cong Li, Lingzhen Li, Xiangli Yangor together with 5 μg of Flag-ATF4 using lipofectamine (Invitrogen). Cells were treated with 25 μM MG115(3) to stabilize ATF4 for 5 hours before harvesting. Isolated whole-cell lysates were immunoprecipitated

(12) Increased Inflammation and Unchanged Density of Synaptic Vesicle Glycoprotein 2A (SV2A) in the Postmortem Frontal Cortex of Alzheimer's Disease PatientsFrontiers in cellular neuroscienceJanuary 1, 2019Metaxas A, Thygesen C, Briting SRR, Landau AM, Darvesh S, Finsen B.(pH 7.4), containing 3,3’-diaminobenzidine (DAB; 0.05%) and H2 O2 (0.01%). Biotin-labeled mouse IgG1 (MG115, Thermo Fisher Scientific), diluted to the same concentration as the primary antibodies, was used for

(13) RNA virus interference via CRISPR/Cas13a system in plantsGenome BiologyDecember 1, 2018Rashid Aman, Zahir Ali, Haroon Butt, Ahmed Mahas, Fatimah Aljedaani, Muhammad Zuhaib Khan, Shouwei Ding, Magdy Mahfouzprotease inhibitors, PMSF, leupeptin, aprotinin, pepstatin, antipain, chymostatin, Na2VO3, NaF, MG132, and MG115. Proteins were separated on a 10% polyacrylamide gel. Immunoblot analysis was carried out using mouse

(14) Behavioural Phenotyping of APPswe/PS1δE9 Mice: Age-Rrelated Changes and Effect of Long-Term Paroxetine TreatmentPLOS ONENovember 4, 2016Louise Ørum Olesen, Elena V. Bouzinova, Maurizio Severino, Mithula Sivasaravanaparan, Jørgen Bo Hasselstrøm, Bente Finsen, Ove Wiborgdiluted 1:500, first 30 min at RT and next o.n. at 4°C, using biotinylated isotype IgG1 diluted 1:50 (MG115, Invitrogen) as control. Next day the sections were rinsed in TBS/T, blocked in TBS/Methanol/H202 (8:1:1)

(15) Different Degree in Proteasome Malfunction Has Various Effects on Root Growth Possibly through Preventing Cell Division and Promoting Autophagic VacuolizationPLoS ONESeptember 21, 2012Xianyong Sheng, Qian Wei, Liping Jiang, Xue Li, Yuan Gao, Li WangLysoTracker Green and FM4–64 were purchased from Invitrogen. The stock solutions of MG132 (40 mM), MG115 (40 mM), FM4–64 (500 µg ml−1) and LysoTracker Green (1 mM) were prepared using dimethylsulfoxide (DMSO)

(16) NPR3 and NPR4 are receptors for the immune signal salicylic acid in plantsNatureJune 1, 2012Zheng Qing Fu et al.0.1% Triton X-100, 0.2% Nonidet P-40, and inhibitors: 50 μg/ml TPCK, 50 μg/ml TLCK, 0.6 mM PMSF, 40 μM MG115). The xtracts were then pre-cleared with 50 μl of Dynabeads Protein G (Invitrogen). After 1 μl of anti-GFP

(17) Geldanamycin selectively targets the nascent form of ERBB3 for degradationCell Stress and ChaperonesSeptember 1, 2010Candice S. Gerbin, Ralf Landgraffor degradation (Invitrogen). Twenty-four hours after transfection, cells were treated with 50 μM MG115 for 3 h and with 50 mM phenylarsine oxide (PAO) for the last 30 min of MG115 treatment. Cells were then

(18) Activation of proteasome by insulin-like growth factor-I may enhance clearance of oxidized proteins in the brainMechanisms of Ageing and DevelopmentJanuary 14, 2010Elizabeth Crowe, Christian Sell, Jeff D. Thomas, Gregg J. Johannes, Claudio Torresmethods 2.1.1 Materials Media and serum were purchased from Life Technologies, Inc. (Carlsbad, CA). Epoxomicin, MG115, N-succinyl-LLVY-AMC, Boc-LRR-AMC, Z-LLE-AMC, U0126, LY29402, rapamycin, antibodies against α (PW 8195),

(19) Activation of PERK kinase in neural cells by proteasome inhibitor treatmentJournal of NeurochemistryJanuary 1, 2010Le Zhang, Philip J. Ebenezer, Kalavathi Dasuri, Annadora J. Bruce-Keller, Sun Ok Fernandez-Kim, Ying Liu, Jeffrey N. Kellersupplies were obtained from GIBCO Life Sciences (Gaithersburg, MD, USA). Proteasome inhibitor MG132 and MG115 were obtained from Calbiochem (San Diego, CA). Velcade was purchase from ChemieTek (Indianapolis, IN).

(20) MDM2 regulates dihydrofolate reductase activity through monoubiquitinationCancer researchMay 1, 2008Maguire M, Nield PC, Devling T, Jenkins RE, Park BK, Polański R, Vlatković N, Boyd MT.Lipofectamine 2000 (Invitrogen) was used instead as a delivery agent at 2.5 μL/μg of DNA. When indicated, MG115 (10 μmol/L) was added 3 h before harvesting. Small interfering RNA (siRNA) was delivered to cells using

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Mouse IgG1 Biotin Isotype Control from Thermo Fisher Scientific

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Mouse IgG1 Biotin Isotype Control from Thermo Fisher Scientific

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