Western blot analysis of Phospho-K-Ras (Ser89) in extracts of various cell lines. Samples were incubated with Phospho-K-Ras (Ser89) Polyclonal antibody (Product # PA5-117257) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Jurkat cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 180s.