HA tag was immunoprecipitated in 5 µg C terminal HA Tag fusion protein lysate with HA Tag Recombinant Monoclonal Antibody (Product # MA5-42442) at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate. Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 60 mins at room temperature. Lane 1: HA Tag fusion protein lysate (input). Lane 2: IP in HA Tag fusion protein lysate. Lane 3: IP in HA Tag fusion protein lysate. Lane 4: Rabbit IgG instead of in HA Tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.