Immunohistochemical analysis of A-Raf in human liver using a Recombinant Monoclonal antibody (Product # MA5-42891) at a dilution of 1:100. Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol..

Western blot analysis of A-Raf in extracts of various cell lines. Samples were incubated with A-Raf Monoclonal antibody (Product # MA5-42891) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.