Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb 3108L from Cell Signaling Technology

Product Specs
ItemPhospho-Smad2 (Ser465/467) (138D4) Rabbit mAb
CompanyCell Signaling Technology
Price $936.00 Inquire
Catalog Number3108L
Quantity300 µl
ApplicationsW, W-S
ReactivityH, M, R, Mi
TargetSmad2 (Ser465/Ser467) phosphate
ModificationsSmad2 (Ser465/Ser467) phosphate
HostRabbit
NCBI Gene AliasesCHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2
ClonalityMonoclonal
NCBI Full Gene NameSMAD family member 2
Research AreaDevelopmental Biology
IsotypeIgG
Clone138D4
Molecular Weight60

Questions and Answers

What specific applications has the Phospho-Smad2 (Ser465/467) antibody been used for in scientific research?

The Phospho-Smad2 (Ser465/467) antibody has been shown to work in applications such as Western blotting and immunohistochemistry, particularly in studies investigating the role of TGF-β signaling in various cellular contexts.

Can this antibody be used to study TGF-β signaling in immune cells?

Yes, the antibody has been referenced in studies examining TGF-β activation mechanisms in immune cells, such as the research by Dedobbeleer et al. (2017), which highlights the role of TGF-β in class-switch recombination in human B lymphocytes.

Is there evidence supporting the use of this antibody in cancer research?

Yes, the antibody has been cited in studies related to cancer, including investigations into the role of TGF-β signaling in tumor progression and metastasis, as seen in the context of cardiac hypertrophy and fibrosis in various cancer models.

How does the antibody contribute to understanding the mechanisms of fibrosis?

The Phospho-Smad2 (Ser465/467) antibody is crucial for detecting phosphorylated Smad2, a key mediator in the TGF-β signaling pathway, which has been implicated in fibrotic processes, as demonstrated in studies exploring cardiac fibrosis and the effects of therapeutic agents like curcumin.

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Targeting the αvβ5 Integrin Modifies the TGF-β-Rich Tumor Microenvironment of Pancreatic CancerCancer ResAugust 4, 2026Kodai Suzuki, Yuki Kunisada, Yukihito Kuroda, Hotaka Kawai, Tatiana Hurtado de Mendoza, Evangeline S. Mose, Shingo Eikawa, Henri Havia, Tero A. H. Järvinen, Caisheng Lu, et al.performed with 1% BSA. The sections were stained with phospho-SMAD2 rabbit mAb (clone; 138D4; Cat# 3108, RRID: AB_490941; Cell signaling, Danvers, MA), anti-mouse CD8 Ab (clone EPR21769; Cat# ab217344; Abcam),

(2) The chicken retrovirus-like gene ENS-1/ERNI and Soprano LTR are involved in primordial germ cell developmentiScienceJuly 17, 2026Yuya Okuzaki, Akane Kawaguchi, Yumi Ozaki, Takeo Uemura, Daisuke Saito, Ken-ichi NishijimaAB_3751491 Phospho-SMAD2 (Ser465/467) (138D4) Rabbit Monoclonal Antibody,Cell Signaling Technology,Cat#3108; RRID: AB_490941 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody,Cell Signaling Technology,Cat#9101;

(3) Iron Deficiency-Induced Hair Loss Is Associated with ROS-Mediated Disruption of Wnt/β-Catenin SignalingNutrientsJuly 15, 2026Sang-Ah Kwon, So Young Bu, Yeon-Hee Kim, Joo Weon Lim, Christopher D. Vulpe, Seung-Min LeeMinneapolis, MN, USA), anti-smad2 (Santa Cruz-sc101153, Dallas, TX, USA), anti-phospho-smad2 (Cell signaling-3108, Danvers, MA, USA), anti-cleaved caspase-3 (Cell signaling-9661, Dancers, MA, USA), anti-cleaved caspase-9

(4) Endothelial epigenetic senescence driven microglial activation mediates cardio-retinal neuroinflammation in heart failureTheranosticsJuly 13, 2026Mengdan Wang, Shuo Zhang, Tursunjan Aziz, Shiyao Zhang, Xiao Wu, Gang Li, Yan Wangantibodies: TGFβ2 (Proteintech, 19999-1-AP; 1:1000; or Invitrogen, MA5-37505; 1:1000), p-SMAD2 (CST, 3108; 1:1000), SMAD2 (CST, 3103; 1:1000), SMAD2/3 (CST, 8685; 1:1000), p-SMAD1/5/9 (CST, 13820; 1:1000),

(5) Pancreatic Adenocarcinoma Up-Regulated Factor Promotes Epithelial-Mesenchymal Transition and Lung Metastasis in Hepatocellular CarcinomaInt J Mol SciJuly 12, 2026Jeong-Ran Park, Hyejun Ham, Miso Lee, Jae Ho Seo, Dong-Keon LeeMEK1/2 (cat# 9122S; 1:1000), p-ERK1/2 (cat# 9101S; 1:1000), ERK1/2 (cat# 9102S; 1:1000), p-SMAD2 (cat# 3108S; 1:1000), p-SMAD3 (cat# 9520S; 1:1000), SMAD2/3 (cat# 8685S; 1:1000), and E-cadherin (cat# # 3195S;

(6) Aspirin prevents postoperative peritoneal adhesions by inhibiting the TGF‑β1/Smad signaling pathway in ratsMolecular Medicine ReportsJune 29, 2026Siqi Yan, Yuan Ren, Bingyan Liu, Pai Xiong, Yimin SunAbcam), rabbit anti-α-SMA (1:2,000; cat. no. ab32575; Abcam), rabbit anti-p-Smad2 (1:1,000; cat. no. 3108; Cell Signaling Technology, Inc.), rabbit anti-Smad2 (1:500; cat. no. 1641-1; Epitomics; Abcam), rabbit

(7) Aortic asprosin overexpression does not ameliorate disease pathophysiology in a murine model of Marfan syndromeSci RepJune 25, 2026Prithviraj Manohar Vijaya Shetty, Andrea Matzen, Susanne Hille, Sabine Michalewski, Henrike Witthaus, Marie Noormalal, Fady Marcous, Yousef Morcos, Tarik Bozoglu, Wiebke Sommer, et al.ab134047, 1:1,000), β-actin (Sigma-Aldrich, A5441, 1:10,000), phospho-SMAD2 (Cell Signalling Technology, #3108, 1:1,000), phospho-SMAD3 (Cell Signalling Technology, #9520, 1:1,000), SMAD2/3 (Cell Signalling Technology,

(8) Intraflagellar transport protein IFT172 contains a C-terminal ubiquitin-binding U-box-like domain involved in ciliary signalingElifeJune 23, 2026Nevin K Zacharia, Stefanie Kuhns, Niels Boegholm, Anni Christensen, Jiaolong Wang, Narcis A Petriman, Anna Lorentzen, Jindriska L Fialova, Lucie Menguy, Sophie Saunier, et al.tag,Genscript#A00186,1:5000,Dako#P0161,1:1000 IFT172,Santa Cruz#Sc398393,1:200,Agilent#P0447,"1:10,000" p-SMAD2,Cell Signaling#3108 s,1:500,Agilent#P0399,"1:10,000" p-AKTT308,Cell Signaling#2965 s,1:500,Agilent#P0399,"1:10,000" p-AKTS473,Cell Signaling

(9) LIF and Oncostatin M mitigate LPS-induced acute endometritis via distinct immunoregulatory mechanisms in peritoneal macrophages in vivoiScienceJune 19, 2026Marion Ravelojaona, Julie Girouard, Cathy Vaillancourt, Céline Van Themsche, Carlos Reyes-Moreno(Danvers, MA, USA)",#4904 phospho-SMAD2 pS465/467,"Cell Signaling Technologies (Danvers, MA, USA)",#3108 total-SMAD2,"Cell Signaling Technologies (Danvers, MA, USA)",#5359 Mouse monoclonal antibodies targeting

(10) Targeting oncogenic TβRI signaling inhibits androgen-independent prostate cancer growth and metastasisSignal Transduct Target TherJune 17, 2026Per Flodbring Larsson, Alexej Schmidt, Yabing Mu, Guangxiang Zang, Jie Song, Vishnupriya Gajavilli, Junting Tao, Olena Rakhimova, Madelene Ericsson, Karthik Aripaka, et al.transfer onto nitrocellulose membranes and immunoblotting using primary antibodies including pSMAD2 (3108S) and SMAD2 (3103S), pp38 (9211S), p38 (9217S), pAKT (4060S), AKT (2920S), pp65 (3033S), and p65 (8242S),

(11) CD109 Drives Stemness and Chemoresistance in Colorectal Cancer via LRRC8A/AKAP12/PKCα-Mediated STAT3 Activation and WNT SignalingInt J Biol SciJune 4, 2026Rubén A. Bartolomé, Tania Calvo-López, Pablo Otero-Núñez, María Estaras, Asunción Fernández-Barral, Antonio Barbáchano, Issam Boukich, María Cuerda-López, Mª Jesús Fernández-Aceñero, Gorane Rodríguez-Urquirizar, et al.from Santa Cruz Biotechnologies (Dallas, TX, USA). Anti-phospho-STAT3 (#9145), anti-phospho-SMAD2 (#3108), anti-STAT3 (#4904), anti-SMAD2/3 (#8585), anti-CD109 (#24765) and anti-LRRC8A (#24979) for immunoprecipitation

(12) Dietary Quercetin-3-Glucuronide Mitigates Oxidative Stress, Inflammation, and Fibroblast Transition by Regulating Nrf2 and Autophagy in Pulmonary FibrosisJournal of Agricultural and Food ChemistryJune 3, 2026Pei-Rong Yu, Chiao-Yun Tseng, Yu-Hsuan Liang, Yu-Ci Chang, Jing-Hsien Chen, Hui-Hsuan Linfibronectin (sc-8422), and vimentin (sc-373717) from Santa Cruz Biotechnology (Santa Cruz, CA); p-Smad2 (3108t), Smad2 (5339t), p-Smad3 (9520t), and Smad3 (9523t) from Cell Signaling Technology (Danvers, MA), and

(13) The Pathogenic a Disintegrin and Metalloprotease with Thrombospondin Type I Motifs-2 D167N Variant Causes Geleophysic Dysplasia-Like Connective Tissue Changes in MiceThe American Journal of PathologyJune 1, 2026Connie Lin, Divya I. Sivakumar, Ana D. Alcocer, Sophia T. Gavalas, Nandaraj Taye, Deborah E. Seifert, Zerina Balic, Timothy J. Mead, Dirk Hubmacherprocedures, as previously described.27,28 Antigen retrieval was used prior to anti–Phospho-SMAD2 (1:200; #3108; Cell Signaling Technology, Danvers, MA) antibody staining by microwaving for 4 × 1.5 minutes in a microwave

(14) Roles of Msx2 in exogen control: modulating the stem cell niche during the transition from hair shedding to regenerationJournal of Advanced ResearchJune 1, 2026Qi Chen, Wen-Chien Jea, Ting-Xin Jiang, Ping Wu, Chi Zhang, Ji Li, Mingxing Lei, Cheng-Ming Chuong, Ya-Chen Liangantibodies and dilutions were used as follows: SMAD2/3 (1:2000, 8685, Cell Signaling), pSMAD2 (1:2000, 3108, Cell Signaling), Cytokeratin 10 (1:1000, sc-53252, Santa Crutz), GAPDH (1:1000, 60004-1-Ig, Proteintech). Cell

(15) CA19-9 induces microenvironment remodeling in pancreatic ductal adenocarcinomabioRxivMay 28, 2026Jasper Hsu, Hyemin Song, Satoshi Ogawa, Casie S. Kubota, Kristina L. Peck, Emily Jacobs, Lizmarie Garcia-Rivera, Jonathan Zhu, Yuan Sui, Woncheol Jung, et al.(NF-kB) (S536; CST #3033), p65 (NF-kB) (CST #8242), pAkt (S473; CST #4060), AKT (CST #4685), pSMAD2 (CST #3108), SMAD2/3 (CST #8685), pERK (T202/Y204; CST #4370), ERK (CST #4695), hFbln3 (RnD #AF6235), CA19–9 (HuMab-5B1

(16) Hongjam, an edible silkworm-derived food, attenuates steatohepatitis and fibrosis via multi-axis modulation of metabolic stress, inflammation, and fibrogenic signalingFrontiers in NutritionMay 26, 2026Hye-Rin Ahn, Seung-Won Lee, Da-Young Lee, Moon-Young Song, Young-Min Han, Byeong Yeob Jeon, You-Kyung Jang, Eun-Hee Kimorigin and supplier TGF-βRI,Sc-101517,"Rabbit monoclonal, Santa Cruz Biotechnology, Inc" p-SMAD2,#3108,"Rabbit monoclonal, Cell signaling technology, Inc" p-SMAD3,#9520,"Rabbit monoclonal, Cell signaling technology

(17) Recurrent evolution of ligand-binding domain multiplicity fine-tunes TGFβ signaling in vertebratesNat CommunMay 19, 2026Jerome Jatzlau, Michael Trumpp, Julia Kühlwein, Leon Obendorf, Yao Le, Heiner Kuhl, Marco Preussner, Paul-Lennard Mendez, Hendrik Burkert, Wiktor Burdzinski, et al.antibodies: anti-Halo (ProMega; #G9211; monoclonal mouse antibody), anti-pSMAD2 Ser465/467 (Cell Signaling; #3108; monoclonal rabbit antibody), and anti-GAPDH (Cell Signaling; #2118; monoclonal rabbit antibody) were

(18) TGFβ-mediated dural progenitor cell migration into the coronal suture is crucial for preventing craniosynostosisNature CommunicationsMay 19, 2026Peng Chen, Lin Meng, Lin Lan, Jifan Feng, Tingwei Guo, Lu Gao, Hana Hekmat, Calista Ly, Mingyi Zhang, Sa Cha, et al.antibodies: anti-Ki67 (Cell Signaling Technology 9129, 1:100), anti-p-SMAD2 (Cell Signaling Technology 3108, 1:100), anti-TGFβ1 (Santa Cruz sc-130348, 1:50), anti-TGFβ2 (Santa Cruz sc-374658, 1:50), anti-TGFβ3

(19) Helicase A determines the transcription program of TH17 lineage differentiation and autoimmunitySci AdvMay 15, 2026Yanhong Su, Anjun Jiao, Xiaoran Zhang, Yanan Xu, Huanxin Ping, Kun Zhu, Qiaohao Wang, Ning Yuan, Yang Gao, Bomiao Ju, et al.anti-Zap70 antibody (catalog no. 3165, Cell Signaling Technology), anti-p-SMAD2 antibody (catalog no. 3108, Cell Signaling Technology), anti-SMAD2 antibody (catalog no. ab33875, Abcam), anti-p-SMAD3 antibody

(20) Nerandomilast attenuates Pantoea agglomerans-induced hypersensitivity pneumonitis by regulating M1 macrophage polarization and fibroblast activationRespiratory ResearchMay 14, 2026Yujie Shi, Yuming Liu, Zhigang Liu, Ruxuan Chen, Mengqi Wang, Zhiyi Li, Qi Chen, Chi Shao, Xiaohe Li, Honggang Zhou, et al.Affinity",p-PI3K,"AF3241, Affinity" STAT1,"AF6300, Affinity",PI3K,"AF6241, Affinity" p-AKT,"AF0016, Affinity",p-Smad2,"3108, CST" AKT,"AF6261, Affinity",Smad2,"5339, CST" p-P38,"28796-1-AP, Proteintech",p-Smad3,"9520, CST" P38,"14064-1-AP,

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Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb from Cell Signaling Technology

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