Pacific Blue(TM) anti-mouse CD4 100534 from BioLegend

Product Specs
ItemPacific Blue(TM) anti-mouse CD4
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100534
NCBI Gene AliasesL3T4, Ly-4
Conjugate/TagPacific Blue
IsotypeIgG2a, Kappa
Quantity25 µg
HostRat
CloneRM4-5
ReactivityMouse
ApplicationsFlow Cytometry (FC)
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) PLK1 inhibition dampens NLRP3 inflammasome-elicited response in inflammatory disease modelsThe Journal of clinical investigationNovember 1, 2023Baldrighi M, Doreth C, Li Y, Zhao X, Warner E, Chenoweth H, Kishore K, Umrania Y, Minde DP, Thome S, et al.605 (eBioscience, 83-0451-42), B220-APC/cyanine 7 (BioLegend, 103223), CD4–Pacific Blue (BioLegend, 100531), CD8–Pacific Blue (BioLegend, 100725), CD11b–Alexa Fluor 488 (BioLegend, 101217), F4/80–PerCP/cyanine

(2) Protocol for neonatal respiratory syncytial virus infection in mice and immune analysis of infected lungs and bronchoalveolar lavage fluidSTAR ProtocolsSeptember 1, 2023Sandeep Adhikari, Pankaj Baral(1:200),BioLegend,101259; AB_2566568 Anti-mouse CD4 (Clone ID: RM4-5) Pacific Blue (1:200),BioLegend,100531; AB_493374 Anti-mouse CD8a (Clone ID: 53-6.7) PE-Cy7 (1:200),BioLegend,100722; AB_312761 Anti-mouse

(3) Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansionCell stem cellJuly 6, 2023Becker HJ, Ishida R, Wilkinson AC, Kimura T, Lee MSJ, Coban C, Ota Y, Tanaka Y, Roskamp M, Sano T, et al.(M1/70),Thermo Fisher Scientific,Cat# 11-0112-82; RRID:AB_464935 anti-mouse CD4-PB (RM4-5),BioLegend,Cat#100531; RRID:AB_493374 anti-mouse CD8a-PB (53-6.7),BD Biosciences,Cat#558106; RRID:AB_397029 anti-mouse CD45R(B220)-PB

(4) Vector Aided Microenvironment programming (VAMP): reprogramming the TME with MVA virus expressing IL-12 for effective antitumor activityJournal for ImmunoTherapy of CancerApril 28, 2023Laura Seclì, Luigia Infante, Linda Nocchi, Maria De Lucia, Gabriella Cotugno, Guido Leoni, Elisa Micarelli, Irene Garzia, Lidia Avalle, Giulia Sdruscia, et al.553785). Treg: CD45 (eBioscience, clone 30-F11, cat. 45-0451-82), CD4 (BioLegend, clone RM 4–5, cat. 100531), CD8 (BioLegend, clone 53–6.7, cat. 100752), Foxp3 (eBioscience, clone FJK-16s, cat. 17-5773-82). CD8

(5) Cladribine treatment improves cortical network functionality in a mouse model of autoimmune encephalomyelitisJ NeuroinflammationNovember 8, 2022Christina B. Schroeter, Leoni Rolfes, K. S. Sophie Gothan, Joel Gruchot, Alexander M. Herrmann, Stefanie Bock, Luca Fazio, Antonia Henes, Venu Narayanan, Steffen Pfeuffer, et al.Flow cytometry,Flow cytometry,Flow cytometry,Flow cytometry CD3,Mouse,BioLegend,100218 CD4,Mouse,BioLegend,100531 CD8a,Mouse,BioLegend,100730 CD11a,Mouse,BioLegend,153103 CD11b,Mouse/human,BioLegend,101263 CD11c,Mouse,BioLegend,117306 CD25,Mouse,BioLegend,101915 CD44,Mouse,BioLegend,103049 CD45,Mouse,BioLegend,103116 CD45R/B220,Mouse,BioLegend,103211 CD49d,Mouse,BioLegend,103627 CD62L,Mouse,BioLegend,104437 CD69,Mouse,BioLegend,104521 NK1.1,Mouse,BioLegend,108710 Antibodies

(6) Platelet depletion does not alter long-term functional outcome after cerebral ischaemia in miceBrain, Behavior, & Immunity - HealthOctober 1, 2022Rebecca D. Steubing, Fabian Szepanowski, Christina David, Ayan Mohamud Yusuf, Stine Mencl, Anne-Kathrin Mausberg, Harald F. Langer, Manuela Sauter, Cornelius Deuschl, Michael Forsting, et al.using the following antibodies: CD45 (# 103115, BioLegend, USA), CD3 (# 100203, BioLegend, USA), CD4 (# 100534, BioLegend, USA), CD8 (# 100751, BioLegend, USA), CD11b (# 550993, BD Biosciences, USA), Ly6G (# 553128,

(7) AMP-activated protein kinase alpha1 promotes tumor development via FOXP3 elevation in tumor-infiltrating Treg cellsiScienceJanuary 21, 2022An J, Ding Y, Yu C, Li J, You S, Liu Z, Song P, Zou MH.).,BioLegend,100237; RRID:AB_2562039 Flow cytometry: Pacific blue anti-mouse CD4 antibody (RM4-5),BioLegend,100531; RRID:AB_493374 Flow cytometry: PE/Cyanine 7 anti-mouse CD4 antibody (GK1.5),BioLegend,100422; RRID:AB_312707 Flow cytometry

(8) Maternal regulation of biliary disease in neonates via gut microbial metabolitesNature communicationsJanuary 10, 2022Jee JJ, Yang L, Shivakumar P, Xu PP, Mourya R, Thanekar U, Yu P, Zhu Y, Pan Y, Wang H, et al.anti-mouse CD3 (clone: 17A2, eBioscience, 11-0032-82), Pacific Blue anti-mouse CD4 (clone: RM4-5, BioLegend, 100531), APC anti-mouse CD8a (clone: 53-6.7, eBioscience, 17-0081-81), PE/Cy7 anti-mouse CD25 (clone: 7D4,

(9) CXCL10(+) peripheral activation niches couple preferred sites of Th1 entry with optimal APC encounterCell reportsAugust 10, 2021Prizant H, Patil N, Negatu S, Bala N, McGurk A, Leddon SA, Hughson A, McRae TD, Gao YR, Livingstone AM, et al.RM4-5),Biolegend,Cat# 100548; RRID: AB_11125962 anti-mouse CD4 Pacific Blue (clone RM4-5),Biolegend,Cat# 100531; RRID: AB_493375 anti-mouse CD4 PE (clone RM4-5),Biolegend,Cat# 100512; RRID: AB_312715 anti-mouse

(10) Interrogating the small intestine tuft cell – ILC2 circuit using in vivo manipulationsCurrent ProtocolsMarch 26, 2021Claire E. O'Leary, Xiaogang Feng, Victor S. Cortez, Richard M. Locksley, Christoph SchneiderLineage cocktail 1:300 all NK1.1 Biolegend 108722 DX5 Biolegend 108918 CD3 Biolegend 100214 CD4 Biolegend 100531 CD8α Biolegend 100725 Ter119Biolegend 116232 F4/80 Biolegend 123124 CD11b Biolegend 101224 CD11c Biolegend

(11) Transcriptional Diversity and Niche-Specific Distribution of Leukocyte Populations during Staphylococcus aureus Craniotomy-Associated Biofilm InfectionJournal of immunology (Baltimore, Md. : 1950)February 15, 2021Aldrich AL, Horn CM, Heim CE, Korshoj LE, Kielian T.

(12) The TGF-β superfamily cytokine Activin-A is induced during autoimmune neuroinflammation and drives pathogenic Th17 cell differentiationImmunityFebruary 9, 2021Wu B, Zhang S, Guo Z, Bi Y, Zhou M, Li P, Seyedsadr M, Xu X, Li JL, Markovic-Plese S, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies Pacific Blue anti-mouse CD4,Biolegend,Cat# 100531 RRID:AB_493646 PE anti-mouse IL-10,Biolegend,Cat# 505008RRID:AB_315362 FITC anti-mouse IL-10,Biolegend,Cat#

(13) Spleen plays a major role in DLL4-driven acute T-cell lymphoblastic leukemiaTheranosticsJanuary 1, 2021Xiong H, Mancini M, Gobert M, Shen S, Furtado GC, Lira SA, Parkhurst CN, Garambois V, Brengues M, Tadokoro CE, et al.For mouse immunohistochemistry, the following antibodies were used: anti-CD4 Alexa 647 (Biolegend #100531; clone: RM4-5), anti-CD8 Alexa 488 (Biolegend #100723; clone: 53-6.7), anti-CD4 615 (eBioscience #42-0042-80;

(14) PRMT5 Promotes Cyclin E1 and Cell Cycle Progression in CD4 Th1 Cells and Correlates With EAE SeverityFrontiers in immunologyJanuary 1, 2021Amici SA, Osman W, Guerau-de-Arellano M.15 min at 4°C. Primary antibodies included: CD3 (BioLegend, 100334, clone 145-2C11), CD4 (BioLegend, 100531 or eBioscience, 12-0042-85, clone RM4-5). Samples were run on a FACSCalibur with DxP multicolor upgrades

(15) Obesity Shapes Metabolism in the Tumor Microenvironment to Suppress Anti-Tumor ImmunityCellDecember 23, 2020Ringel AE, Drijvers JM, Baker GJ, Catozzi A, García-Cañaveras JC, Gassaway BM, Miller BC, Juneja VR, Nguyen TH, Joshi S, et al.

(16) A Single Immunization with Nucleoside-Modified mRNA Vaccines Elicits Strong Cellular and Humoral Immune Responses against SARS-CoV-2 in MiceImmunityOctober 13, 2020Laczkó D, Hogan MJ, Toulmin SA, Hicks P, Lederer K, Gaudette BT, Castaño D, Amanat F, Muramatsu H, Oguin TH 3rd, et al.1:100 in 0.1% BSA in PBS: anti-CD3 APC-Cy7 (BD Biosciences #557596), anti-CD4 Pacific Blue (Biolegend #100531), anti-CD8 PerCP-Cy5.5 (BD Biosciences #551162), anti-PD-1 APC (Biolegend #109112), anti-CD69 BV605

(17) Continued Bcl6 Expression Prevents the Transdifferentiation of Established Tfh Cells into Th1 Cells during Acute Viral InfectionCell reportsOctober 6, 2020Alterauge D, Bagnoli JW, Dahlström F, Bradford BM, Mabbott NA, Buch T, Enard W, Baumjohann D.

(18) STING couples with PI3K to regulate actin reorganization during BCR activationScience advancesApril 1, 2020Jing Y, Dai X, Yang L, Kang D, Jiang P, Li N, Cheng J, Li J, Miller H, Ren B, et al.spleen, and LNs were stained with PE-anti-CD4 (BioLegend, 100408), Pacific Blue-anti-CD4 (BioLegend, 100531), Brilliant Violet 510-anti-CD8a (BioLegend, 100751), APC/Cy7-anti-TCRβ (BioLegend, 109220), 7-AAD (BD

(19) Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunityThe Journal of clinical investigationApril 1, 2020Webb LM, Sengupta S, Edell C, Piedra-Quintero ZL, Amici SA, Miranda JN, Bevins M, Kennemer A, Laliotis G, Tsichlis PN, et al.553087, clone RA3-6B2), CD3 (BioLegend, catalog no. 100334, clone 145-2C11), CD4 (BioLegend, catalog no. 100531 or eBioscience, catalog no. 12-0042-85, clone RM4-5), CD25 (Invitrogen, catalog no. RM6017 or eBioscience,

(20) Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune CellsImmunityMarch 17, 2020Kolev M, West EE, Kunz N, Chauss D, Moseman EA, Rahman J, Freiwald T, Balmer ML, Lötscher J, Dimeloe S, et al.PE (RM4-5),Biolegend,"Cat. #100512, RRID:AB_312715" Anti-mouse CD4 PacBlue (RM4-5),Biolegend,"Cat. #100531, RRID:AB_493374" Anti-mouse CD25 APC (PC61),Biolegend,"Cat. #102012, RRID:AB_312861" Anti-mouse CD45RB

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11/25/2015CD4 Pacific Blue Antibody Review Michaela RekowSpleens from latently infected mice are collected and processed for flow. Identification and quantification of various immune cells are then established.

Pacific Blue(TM) anti-mouse CD4 from BioLegend

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Pacific Blue(TM) anti-mouse CD4 from BioLegend

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