Fig 1: Identification of 3 neutrophil subclusters from lupus kidney. (a) UMAP plot showed 27 cell types in both control and lupus kidney. (b) 3 neutrophil subclusters and B cells were isolated from the whole kidney. (c) Dot plot showed the expression level of features for neutrophils and B cells. Cd79a and Cd79b for B cells, Ly6g for all neutrophils, and Aldh2 and Socs3 for newly identified 2 neutrophil subclusters. (d) Stacked bar plot showed the comparison of the frequencies of 3 neutrophil subclusters and B cells. (e) Flow cytometry analysis validated 3 neutrophil subclusters in kidney. Dead cells were removed by counterstaining fixable viability dye. (f) Dot plots showed the comparison of 3 neutrophil subclusters between control (n = 8) and lupus (n = 8) kidneys. Data represented similar results from at 3 independent experiments. ∗∗∗p < 0.001; ∗∗p < 0.01; ns: no significance.