Citrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb 97272S from Cell Signaling Technology

Product Specs
ItemCitrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb
CompanyCell Signaling Technology
Price $414.00 Inquire
Catalog Number97272S
ClonalityMonoclonal
ReactivityH, M
ApplicationsW, IHC-P
HostRabbit
Quantity100 µl
TargetH3 (Arg17) citrullate;H3 (Arg8) citrullate;H3 (Arg2) citrullate
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Development of motif-specific monoclonal antibodies for global protein citrullination detection with minimal cross-reactivity to homocitrullinationCell Reports MethodsApril 20, 2026Sophia Laposchan, Erik Riedel, Andrew Flatley, Selina Pasquero, Regina Feederle, Chien-Yun Lee[EPR20358-120],not disclosed,"ELISA, Flow Cyt (Intra), ICC, IF, IP, and WB" Cell Signaling Technology,97272,histone H3 (cit R17),rabbit (IgG),monoclonal [E4O3F],synthetic peptide of histone H4 with citrullinated

(2) Citrullinated histone H3 identifies neutrophil extracellular trap formation and correlates with renal disease activity in ANCA-associated vasculitisClin Kidney JApril 6, 2026Natsumi Kamijo, Akiko Mii, Takashi Tani, Rei Nakazato, Arimi Ishikawa, Tetsuya Kashiwagi, Ryuji Ohashi, Masataka Kuwana, Akira Shimizu, Yukinao Sakai, et al.(FFPE) renal biopsy specimens. For H3Cit, two rabbit antibodies, ab281584 (Abcam, Cambridge, UK) and #97272 (Cell Signaling Technology, Danvers, MA, USA), were initially evaluated in preliminary experiments.

(3) Thromboxane receptor activation in dendritic cells mitigates sepsis by suppressing S100a8/a9-mediated neutrophil recruitmentSignal Transduct Target TherMarch 3, 2026Ronglu Du, Ting Pan, Yuhan Wang, Yan Fan, Qian Liu, Xixi Tao, Shumin Guo, Danyang Tian, Roger S.-Y. Foo, Keliang Xie, et al.(Cell Signaling Technology, Cat# 4060S), rabbit monoclonal anti-Citrullinated Histone H3 (Cell Signaling Technology, Cat# 97272), and rabbit polyclonal anti-β-actin (Abclonal, Cat# AC026). The membranes were then incubated with

(4) Modified Dachengqi Decoction ameliorates sepsis-induced lung injury via the gut microbiota-bile acid axisFrontiers in Cellular and Infection MicrobiologyFebruary 6, 2026Lulu Wu, Weihang Peng, Ya Li, Liyuan Yu, Peiying Huang, Ye Ye, Yuchao Feng, Bojun Chen, Li Chenantibodies, VCAM-1 (1:200, ab134047, Abcam), VE-cadherin (1:200, 27956-1-AP, Proteintech), CitH3 (1:200, 97272S, CST), MPO (1:200, AF3667, R&D),phospho-SAPK/JNK(1:200,9255,CST), and phospho-p65(1:500,3033,CST)were

(5) Escherichia coli promotes colorectal cancer metastasis by maintaining enhancer-promoter loops through releasing neutrophil extracellular trapsNature CommunicationsFebruary 3, 2026Banglun Pan, Yuxin Yao, Zhu Zhang, Dongjie Ye, Hao Wu, Xinyu Zhang, Xiaoqian Wang, Nanhong TangLeica TCS SP8 microscope. Primary antibodies used in this study: anti-CitH3 (1:500, catalog number: 97272, clone name: E4O3F, Cell Signaling Technology), anti-E-Cadherin (1:400), anti-F4/80 (1:50, catalog number:

(6) STK38-mediated feedback loop regulation of the hedgehog pathway governing tumor heterogeneity in renal papillary carcinomaCell Death & DiseaseJanuary 15, 2026Yifan Du, Xiuyuan Sui, Zeyuan Zheng, Zhengying Zhang, Bin Liu, Yang Bai, Yue Zhao, Qingqing Wu, Haodong Wu, Min Zhong, et al.H3 (HUABIO, #M1309-1), anti-PADI4 (Boster, #PB97272S), anti-citrullinated Histone H3 (Arg17) (CST, #97272), and anti-β-catenin (CST, #8480). For Hh pathway components, the Hedgehog Signaling Antibody Sampler Kit

(7) S100A8/S100A9 through PAD4 activation of neutrophil extracellular traps promotes granulomatous lobular mastitisFront ImmunolDecember 16, 2025Tingting Zhu, Hao Yu, Wei Wang, Yulu Sun, Shengjia Wang, Dongwen Ma, Yongzhong Yaoab288715); anti-beta actin (Abcam, ab8226); anti-citrullinated histone H3 (Cell Signaling Technology, #97272); anti-myeloperoxidase (MPO) (Proteintech, 66177-1-Ig); and anti-PAD4 (Proteintech, 17373-1-AP). According

(8) Nicotinamide Attenuates Complement and Coagulation Pathways and Resultant Renal FibrosisFASEB JDecember 15, 2025Saori Kin, Yuji Oe, Taku Obara, Emiko Sato, Nobuyuki Takahashi, Mariko Miyazaki, Tetsuhiro TanakaCD68 (1:2000, #ab125212, Abcam, Cambridge, UK), anti‐rabbit citrullinated histone H3 (CitH3, 1:2000, #97272, Cell Signaling Technology, Danvers, MA), and anti‐human myeloperoxidase (MPO) polyclonal antibody (1:5000,

(9) Neutrophil extracellular traps drive osteoporosis via NCF2-dependent signaling: integrated transcriptomics with mechanistic validationRedox report : communications in free radical researchDecember 1, 2025Guo X, Wang L, Chen S, Sun C, Qin J, Liu Q, Gan Y, Li Y, Jin Z, Wei X, et al.of positive staining. IF method: For immunofluorescence staining, CitH3 (Cell Signaling, Rabbit mAb #97272), NE (Thermo Fisher, PA5-88916) and MPO (Thermo Fisher, MPO-101AP) were used as primary antibodies.

(10) Nicotinamide Attenuates Complement and Coagulation Pathways and Resultant Renal FibrosisFASEB JDecember 1, 2025Saori Kin, Yuji Oe, Taku Obara, Emiko Sato, Nobuyuki Takahashi, Mariko Miyazaki, Tetsuhiro TanakaCD68 (1:2000, #ab125212, Abcam, Cambridge, UK), anti‐rabbit citrullinated histone H3 (CitH3, 1:2000, #97272, Cell Signaling Technology, Danvers, MA), and anti‐human myeloperoxidase (MPO) polyclonal antibody (1:5000,

(11) Effects and the mechanism of pine pollen polysaccharides on diabetic wound healing in vitro and in vivoRegenerative TherapyDecember 1, 2025Lan Chen, Lifang Zhu, Yu Cao(#AF6285, Beyotime; 1:1000), anti-Bax (#AB026; Beyotime; 1:1000), anti-citrullinated H3 (Cit-H3) (#97272; CST, Danvers, MA, USA; 1:1000), anti-H3 (#9715; CST; 1:1000), anti-peptidylarginine deiminase 4 (PAD4)

(12) Emodin Alleviates Sepsis‐Induced Multiorgan Damage by Inhibiting NETosis through Targeting Neutrophils BCL‐10Advanced ScienceNovember 1, 2025Xiaolong Xu, Yumeng Yan, Meiling Zheng, Mina Zhang, Tengfei Chen, Zhicheng Qu, Yinglu Bai, Shuwen Zhang, Chunming Zhao, Yifan Shi, et al.from Thermo Scientific (MA, USA). Anti‐NE antibody (89241), MPO rabbit mAb (14569), CitH3 rabbit mAb (97272), BCL‐10 rabbit mAb (4237), p‐IKKα/β rabbit mAb (2697), p‐IκBα rabbit mAb (2859), p‐NF‐κB p65 rabbit

(13) Indoleamine 2,3-dioxygenase-galectin-3 fusion protein remodels inflammatory and fibrotic programs, ameliorating imiquimod-induced psoriasisNPJ Biomed InnovOctober 22, 2025Jennifer A. Simonovich, Sabrina L. Macias, Mingqi Zhou, Marija Portelli, Isha Verma, Arun Wanchoo, Alexander J. Kwiatkowski, Eric Y. Helm, Tran B. Ngo, Shamima Islam, et al.(Cell Signaling Technologies, cat. no. 19589 s, 1:150), Citrullinated histone 3 (Cell Signaling Technologies, cat. no. 97272 s, (Arg17) (E4O3F), 1:500), CD8 (Cell Signaling Technologies, cat. no. 98941 s, D4W2Z, 1:50), CD32 (Abcam,

(14) Engineered Dual‐Function Antibody‐Like Proteins to Combat SARS‐CoV‐2‐Induced Immune Dysregulation and InflammationAdvanced ScienceJuly 6, 2025Yizhuo Wang, Chenwu Bai, Kerui Hou, Zhen Zhang, Ming Guo, Xin Wang, Fan Yang, Xin Liusequential staining with anti‐MPO (Thermo Fisher Scientific, PA5‐16672, 1:200) and anti‐CitH3 (CST, 97272, 1:800) antibodies. TSA‐647 and TSA‐488 (Thermo Fisher Scientific, B40936, B40932) were used for TSA

(15) Mechanism of Neutrophil p90RSK-Nrf2 Signaling Pathway in AtherosclerosisBalkan Med JJuly 1, 2025Jiawen Li, Lei Wang, Xiao Liang, Xiaoxia LiBSA for 30 min. Next, cells were incubated with citrullinated histone h3 (CitH3) primary antibody (97272S, 1:300, Cell Signaling Technology, Danvers, MA, USA), MRP8/14 primary antibody (1:100, 6279-RBM5-P1,

(16) Intra-Arterial Deoxyribonuclease Therapy Improves Stroke Outcomes in Aged MiceCNS neuroscience & therapeuticsJune 1, 2025Yin J, Wu M, White J, StClair E, Mocco J, Waters MF.antibodies: anti‐Ly6G (BD Bioscience, #551459), anti‐Citrullinated Histone H3 (CitH3, Cell Signaling, #97272), anti‐Neutrophil Elastase conjugated with Alexa Fluor 555 (NE, Cell Signaling, #39680) anti‐Vascular

(17) Neutrophil-to-lymphocyte ratio predicts a poor prognosis for penile cancer with an immunosuppressive tumor microenvironmentFront ImmunolApril 16, 2025Xingliang Tan, Yanjun Wang, Yiqi Yu, Runhao Zheng, Jing Li, Shaohua Chen, Qingling Xie, Shengjie Guo, Chichen Zhang, Xinpei Deng, et al.ab89254), myeloperoxidase (MPO) (CST, #14569), TGF-β (CST, #3709), TIM3 (CST, #75743) and (citH3) (CST, #97272). The visualization of NETs was performed according to MPO and citH3 staining (31). CD66b/CXCR2 and

(18) Thrombo-pathologic features and prognosis of acute ischemic stroke patients treated with remedial stent implantationFrontiers in neurologyJanuary 1, 2025Kang T, Dai L, Yan H, Tian W, Wang Y, Wang C, Li Y, Zhao D.66250-1-Ig, 1:200), VWF (Servicebio, GB11020, 1:1,000), and marker of NETs—Citrullinated Histone H3 (CitH3, 97272, CST, 1: 1,000). It is to be noted that the staining of CitH3 is primarily located in the nucleus, but

(19) Treg and neutrophil extracellular trap interaction contributes to the development of immunosuppression in sepsisJCI insightJune 18, 2024Shi Y, Wu D, Wang Y, Shao Y, Zeng F, Zhou D, Zhang H, Miao C.the primary antibody Foxp3 (1:50, Santa Cruz, 2A11G9) and CitH3 (1:1,000, Cell Signaling Technology, 97272S) were applied to the tissue sections overnight at 4°C. Following this, an anti-rabbit HRP antibody was

(20) Multimodal immune phenotyping reveals microbial-T cell interactions that shape pancreatic cancerCell reports. MedicineFebruary 20, 2024Li Y, Chang RB, Stone ML, Delman D, Markowitz K, Xue Y, Coho H, Herrera VM, Li JH, Zhang L, et al.(SP206),Abcam,ab227670 Rabbit monoclonal anti-human citrullinated histone H3 (E4O3F),Cell Signaling Technology,97272S Mouse monoclonal anti-human CK19 (A53-B/A2.26),Ventana,760-4281; RRID:AB_2335655 Rabbit monoclonal

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Citrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb from Cell Signaling Technology

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Citrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb from Cell Signaling Technology

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