Fig 1: Levels of autophagy and oxidative stress in hearts treated with different nanocarriers in the cardioplegic solution. Quantified results of Beclin 1 (A) and p22phox protein levels in hearts treated with CNN (B). Quantified results of Beclin 1 (C) and p22phox protein levels in hearts treated with CNN-NS (D). Anti-vinculin antibodies were used for the loading control. Values are shown as mean ± SD of O.D. and are expressed as folds to NIH. n = 4–5. * Adjusted p = 0.0372; # adjusted p = 0.0102. Blots were selectively cropped to display only pertinent representative images (vertical dotted lines). The original gels and blots are available for viewing in the Supplementary Materials.