Pacific Blue(TM) anti-mouse CD3 100213 from BioLegend

Product Specs
ItemPacific Blue(TM) anti-mouse CD3
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number100213
NCBI Gene AliasesCtg-3, Ctg3, T3g
ReactivityMouse
IsotypeIgG2b, Kappa
HostRat
Quantity25 µg
Clone17A2
ApplicationsFlow Cytometry (FC)
Conjugate/TagPacific Blue
Antibody TypePrimary
NCBI Full Gene NameCD3 antigen, gamma polypeptide
Concentration0.50 mg/mL
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenγδTCR-positive T-T hybridoma D1
ClonalityMonoclonal
TargetCD3

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Bioresponsive and immunotherapeutic nanomaterials to remodel tumor microenvironment for enhanced immune checkpoint blockadeBioactive MaterialsFebruary 1, 2024Tianxu Fang, Xiaona Cao, Li Wang, Mo Chen, Yueyang Deng, Guojun ChenCat no. 105008); T cells activation and infiltration: CD3 (BioLegend, Pacific Blue-labeled, Cat no.100214), CD4 (BioLegend, APC-labeled, Cat no.100412), CD8 (BioLegend, PE-labeled, Cat no. 140408); M2 macrophages:

(2) Recombinant human DNase-I improves acute respiratory distress syndrome via neutrophil extracellular trap degradationJournal of Thrombosis and HaemostasisSeptember 1, 2023Abbas Jarrahi, Hesam Khodadadi, Nicholas S. Moore, Yujiao Lu, Mohamed E. Awad, Evila L. Salles, Kumar Vaibhav, Babak Baban, Krishnan M. Dhandapaniwere stained with anti-Ly6G (Cat#127625; BioLegend), anti-F4/80 (Cat#123116; BioLegend), anti-CD3 (Cat#100214; BioLegend), anti-CD4 (Cat#100432; BioLegend), anti-CD8 (Cat#140416; BioLegend), anti-CD19 (Cat#551001;

(3) Intrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cellsScience advancesApril 28, 2023Rivera CE, Zhou Y, Chupp DP, Yan H, Fisher AD, Simon R, Zan H, Xu Z, Casali P.BioLegend), DCs with APC-Cy7–anti-CD11c mAb (117323, BioLegend), and T cells with Pacific Blue–anti-CD3 mAb (100214, BioLegend) (table S8). Human B cells were stained with PE-Cy7–anti-human-CD19 mAb (60-0199, Tonbo Biosciences),

(4) Gut microbiota induces hepatic steatosis by modulating the T cells balance in high fructose diet miceScientific reportsApril 24, 2023Zhou X, Zhang X, Niu D, Zhang S, Wang H, Zhang X, Nan F, Jiang S, Wang B.cells. Collected liver mononuclear cells were surface-stained with PB450 anti-mouse CD3 (BioLegend, cat. 100214) and BV605 anti-mouse CD4 (BioLegend, cat. 100451) or PB450 anti-mouse CD3 and APC anti-mouse CD8a (BioLegend,

(5) Controlled release of enhanced cross-hybrid IgGA Fc PD-L1 inhibitors using oncolytic adenovirusesMolecular Therapy - OncolyticsMarch 16, 2023Firas Hamdan, Michaela Feodoroff, Salvatore Russo, Manlio Fusciello, Sara Feola, Jacopo Chiaro, Gabriella Antignani, Francesca Greco, Jeanette Leusen, Erkko Ylösmäki, et al.catalog number 121626, RRID: AB_2572055), and Pacific Blue anti-mouse CD3 (BioLegend, catalog number 100214, RRID: AB_493645). Panel 2 included allophycocyanin (APC) anti-mouse Ly6C (BioLegend, catalog number

(6) Cardiolipin coordinates inflammatory metabolic reprogramming through regulation of Complex II disassembly and degradationScience advancesFebruary 3, 2023Reynolds MB, Hong HS, Michmerhuizen BC, Lawrence AE, Zhang L, Knight JS, Lyssiotis CA, Abuaita BH, O'Riordan MX.and lineage-marker primary-conjugated fluorescent antibodies anti-CD3 Pacific Blue (1:100; BioLegend, 100213), anti-CD19 FITC (1:100; SouthernBiotech, 1575-02), anti-CD11b BV421 (1:100; BioLegend, 101235), and/or

(7) Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1highCD11b+ cells elimination in hepatocellular carcinomaActa Pharmaceutica Sinica BFebruary 1, 2023Jizhou Tan, Ting Liu, Wenzhe Fan, Jialiang Wei, Bowen Zhu, Yafang Liu, Lingwei Liu, Xiaokai Zhang, Songling Chen, Haibiao Lin, et al.PE/Cyanine7 anti-mouse CD45 (Biolegend, 157613, San Diego, CA, USA), Pacific Blue anti-mouse CD3 (Biolegend, 100213, San Diego, CA, USA), Alexa Fluor® 488 anti-mouse CD11b (ThermoFisher, 53-0112-82, Carlsbad, CA, USA),

(8) VLA-4 suppression by senescence signals regulates meningeal immunity and leptomeningeal metastasiseLifeDecember 9, 2022Li J, Huang D, Lei B, Huang J, Yang L, Nie M, Su S, Zhao Q, Wang Y.dye (Thermo Fisher Scientific, Cat# 65-0866-14), CD45 (BioLegend, Cat# 103132), CD3 (BioLegend, Cat# 100213), CD8 (Thermo Fisher Scientific, Cat# 53-0081-82, 17-0088-42), CD4 (Thermo Fisher Scientific, Cat# 62-00420-80),

(9) Double knockin mice show NF-κB trajectories in immune signaling and agingCell reportsNovember 22, 2022Rahman SMT, Aqdas M, Martin EW, Tomassoni Ardori F, Songkiatisak P, Oh KS, Uderhardt S, Yun S, Claybourne QC, McDevitt RA, et al.PE/Cyanine7 anti-mouse/human CD45R/B220,Biolegend,"Cat#103221, Clone RA3-6B2; RRID:AB_313004" Pacific Blue™ anti-mouse CD3,Biolegend,"Cat#100214, Clone 17A2; RRID:AB_493645" PerCP/Cyanine5.5 anti-mouse CD49b,Biolegend,"Cat#108915, Clone DX5; RRID:AB_1595599" Pacific

(10) A potent, broadly protective vaccine against SARS-CoV-2 variants of concernNPJ vaccinesNovember 12, 2022Wang Z, An J, Liu K, Yu P, Fang X, Li J, Zhu H, Zhu Q, Huang C, Zhang C, et al.panel of flow cytometry antibodies specific for cell surface markers: PB anti-mouse CD3 (BioLegend, Cat.100214), FITC anti-mouse CD4 (BD, Cat.553047) for mouse study or PB anti-human CD3 (BD, Cat.558124), FITC anti-human

(11) An in situ hydrogel-mediated chemo-immunometabolic cancer therapyNature communicationsJuly 2, 2022Wang B, Chen J, Caserto JS, Wang X, Ma M.following antibodies were used: CD45-BV650 (BioLegend 103151, 30-F11, 1:200), CD3-Pacific Blue (BioLegend 100213, 17A2, 1:200), PD-L1-PE (BioLegend 124307, 10 F.9G2, 1:100), Calreticulin-Alexa Fluor 647(R&D Systems

(12) A chimeric thermostable M2e and H3 stalk-based universal influenza A virus vaccineNPJ vaccinesJune 29, 2022Subbiah J, Oh J, Kim KH, Shin CH, Park BR, Bhatnagar N, Seong BL, Wang BZ, Kang SM.µg/mL). After 5 h culture, the cells were stained with mouse anti-CD3-PacificBlue (300x dilution, cat no: 100214, clone 17A2, BioLegend), anti-CD4-biotin (300x dilution, cat no: 13-0042-85, clone RM4-5, eBioscience),

(13) Hypoxia shapes the immune landscape in lung injury and promotes the persistence of inflammationNature immunologyJune 1, 2022Mirchandani AS, Jenkins SJ, Bain CC, Sanchez-Garcia MA, Lawson H, Coelho P, Murphy F, Griffith DM, Zhang A, Morrison T, et al.Biosciences,1:400 CD11b,M1/70,101243,B253527,BV785,Biolegend,1:200 MHC-II,M5.114.15.2,107624,B267551,PerCP,eBioscience,1:200 Epcam,G8.8,118230,B251914,APCCy7 Fire,BioLegend,1:200 CD3,17A2,100244,B198733,BIOTIN,BioLegend,1:200 CD3,17A2,100213,B261416,Pacific Blue,BioLegend,1:200 CD3,17A2,100229,B282101,BV650,BioLegend,1:200 CD3,17A2,100206,B210714,PE,BioLegend,1:200 CD19,6D5,115541,B242632,BV650,BioLegend,1:200 CD19,6D5,115504,B244881,Biotin,BioLegend,1:200 CD19,6D5,115526,B265435,Pacific

(14) ADAR1 masks the cancer immunotherapeutic promise of ZBP1-driven necroptosisNatureJune 1, 2022Zhang T, Yin C, Fedorov A, Qiao L, Bao H, Beknazarov N, Wang S, Gautam A, Williams RM, Crawford JC, et al.(BioLegend, Cat# 141604), anti-CD45 APC-Cy7 (BioLegend, Cat# 103116), anti-CD3 Pacific Blue (BioLegend, Cat# 100214) and anti-CD8 FITC (MBL, Cat# D271-4). RIP-seq Analyses Alignment and filtering of RNAs: Initial

(15) Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axisCell ReportsApril 5, 2022Dahai Wang et al.AB_1877267 CD19-PE (eBio1D3),eBioscience,Cat#12–0193-85; RRID: AB_657662 CD3-Pacific blue (17A2),BioLegend,Cat#100214; RRID: AB_493645 CD3-PE (17A2),BioLegend,Cat#100206; RRID: AB_312663 CD34-FITC (RAM34),eBioscience,Cat#11–0341-85;

(16) PRMT5 Deficiency Enforces the Transcriptional and Epigenetic Programs of Klrg1⁺CD8⁺ Terminal Effector T Cells and Promotes Cancer DevelopmentJournal of immunology (Baltimore, Md. : 1950)January 15, 2022Zheng Y, Chen Z, Zhou B, Chen S, Han L, Chen N, Ma Y, Xie G, Yang J, Nie H, et al.

(17) ACE2 is the critical in vivo receptor for SARS-CoV-2 in a novel COVID-19 mouse model with TNF- and IFNγ-driven immunopathologyeLifeJanuary 13, 2022Gawish R, Starkl P, Pimenov L, Hladik A, Lakovits K, Oberndorfer F, Cronin SJ, Ohradanova-Repic A, Wirnsberger G, Agerer B, et al.100217; RRID:AB_1595597,FC (1:200) Antibody,eFluor450 anti-mouse CD3 (monoclonal rat),BioLegend,Cat# 100213; RRID:AB_493644,FC (1:200) Antibody,PE anti-mouse CD3 (monoclonal rat),BioLegend,Cat# 100205; RRID:AB_312662,FC

(18) Human Cytomegalovirus-IE2 Affects Embryonic Liver Development and Survival in Transgenic MouseCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Zhang X, Jiang S, Zhou X, Yu Z, Han S, Nan F, Qiao H, Niu D, Wang Z, Niu J, et al.for staining on the cell surface. The antibodies used were as follows: anti-CD3–Pacific Blue (cat. 100214; Biolegend), anti-CD4–Brilliant Violet 605 (cat. 100451; Biolegend), and anti-CD8–Allophycocyanin (cat.

(19) Nociceptor neurons promote IgE class switch in B cellsJCI insightDecember 22, 2021Mathur S, Wang JC, Seehus CR, Poirier F, Crosson T, Hsieh YC, Doyle B, Lee S, Woolf CJ, Foster SL, et al.Cells from spleen tissue samples were stained for surface markers with anti-CD3–PacBlue (BioLegend 100214), anti-PD1–FITC (BioLegend 135214), anti-B220–PerCP 710 (eBioscience 46-0452-80), anti-B220–PE (BioLegend

(20) Resolving the immune landscape of human prostate at a single-cell level in health and cancerCell reportsDecember 21, 2021Tuong ZK, Loudon KW, Berry B, Richoz N, Jones J, Tan X, Nguyen Q, George A, Hori S, Field S, et al.(FITC),eBioscience,Cat#11-0081-82; clone 53-6.7; RRID: AB_464915 anti-mouse CD3 (Pacific Blue),BioLegend,Cat#100214; clone 17A2; RRID: AB_493645 Flash Phalloidin 488,BioLegend,Cat#424201 Hoechst 33258,Biotium,Cat#40044 DAPI

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(1)

7/28/2016Staining Mouse WBC With Anti-CD3 Pacific Blue Adam GuessThis antibody was used as a T cell marker in a hematopoietic lineage panel. It was chosen because it is a commonly used clone, Pacific Blue is bright enough to fully resolve positive cells, and ... Read Review
(1)

10/26/2015Anti Mouse CD3 PB for Flow Cytometry Yanxia CaoI used this to identify the CD3+ T Cell population in the mouse spleen to use for downstream research.

Pacific Blue(TM) anti-mouse CD3 from BioLegend

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Pacific Blue(TM) anti-mouse CD3 from BioLegend

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