Indirect immunostaining of PFA fixed mouse spleen section with Guinea pig anti-Vcam1 (cat. no. HS-470 004, dilution 1 : 500; red). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval (10mM citrate, pH6.0, overnight at 60°C) has been applied before staining.

Immunohistochemical staining of formalin fixed paraffin embedded tissue sections of A: mouse spleen and B: human tonsil with Guinea pig anti-Vcam1 (cat. no. HS-470 004; 1 : 500). The Guinea pig anti-Vcam1 antibody does not cross-react with human Vcam1 and is suited to distinguish murine from human Vcam1 expression in humanized mouse models. Nuclei have been visualized by haematoxylin staining (blue).

Immunohistochemical staining of formalin fixed paraffin embedded liver sections of left image: a non-infected mouse and right image: a Toxoplasma (T.) gondii infected mouse with Guinea pig anti-Vcam1 (cat. no. HS-470 004; 1 : 500). Strong Vcam1 expression is detected in the T. gondii infected mouse liver. Nuclei have been visualized by haematoxylin staining (blue).

Immunohistochemical staining of formalin fixed paraffin embedded tissue sections of A: syngeneic mouse breast cancer model and B: human colon carcinoma xenograft mouse model (Colo 205) with Guinea pig anti-Vcam1 (cat. no. HS-470 004; 1 : 500). Strong Vcam1 expression is detected in the syngeneic model but not in the human xenograft model, where weak Vcam1 expression is limited to murine endothelial cells. Nuclei have been visualized by haematoxylin staining (blue). Tissues were kindly provided by Charles River Discovery Research Services Germany GmbH.