Indirect immunostaining of PFA fixed rat hippocampus neurons with rat anti-RIM 1/2 antibody (cat. no. 140 217, dilution 1 : 500, red), and rabbit anti-Map2 antibody (cat. no. 188 002, dilution 1 : 1000, green). Nuclei have been visualized by DAPI staining (blue).

Indirect immunostaining of PFA fixed mouse hippocampus section (CA3) with rat anti-RIM 1/2 antibody (cat. no. 140 217, dilution 1 : 100, red) and rabbit anti-Piccolo antibody (cat. no. 142 113, dilution 1 : 500, green). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval at pH 6.0 has been applied before immunostaining.

Indirect immunostaining of PFA fixed mouse cerebellum section with rat anti-RIM 1/2 antibody (cat. no. 140 217, dilution 1 : 100, red) and rabbit anti-Piccolo antibody (cat. no. 142 113, dilution 1 : 500, green). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval at pH 6.0 has been applied before immunostaining.

Indirect immunostaining of PFA fixed rat hippocampus section (dentate gyrus) with rat anti-RIM 1/2 antibody (cat. no. 140 217, dilution 1 : 100, red) and rabbit anti-Piccolo antibody (cat. no. 142 113, dilution 1 : 500, green). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval at pH 6.0 has been applied before immunostaining.