Immunohistochemistry: RPE65 Antibody (7B10) [NBP3-15242] - IHC image of RPE65 antibody diluted at 1:100 and staining in paraffin-embedded human eye tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.