Fig 2: TRIM24 is selectively expressed in HNSCC cells and negatively regulates STAT1.a Volcano plot showing top 100 upstream regulators based on Ingenuity pathway analysis of RNA sequencing data from oral tumour bearing Stat1+/+ and Stat1−/− mice (n = 4 per group). b Predicted mechanistic network analysis showing transcriptional targets of TRIM24 based on Ingenuity pathway analysis of RNA sequencing data from oral tumour-bearing Stat1+/+ and Stat1−/− mice. Genes in red are upregulated and blue are downregulated in the RNA sequencing data set. c Correlation of TRIM24 expression levels with STAT1 expression in tumours from HNSCC patients samples acquired from The Cancer Genome Atlas. Significance determined by Pearson correlation coefficient. d Gene expression of Trim24 determined by RT-qPCR in CAL27 cells treated with scramble or Stat1 siRNA for 72 h. e TRIM24 protein expression by CAL27 cells treated with scramble or Stat1 siRNA for 72 h, determined by flow cytometry. f Gene expression of TRIM24 in tumours of HNSCC tumour-bearing mice orthotopically injected with the LY2 cell line. g TRIM24 protein expression, determined by flow cytometry, in HNSCC cells of mice orthotopically injected with the LY2 cell line. h, j Gene expression of Trim24 and Stat1 determined by RT-qPCR in (H) CAL27 and (J) UMSCC22A cells treated with scramble or Trim24 siRNA for 48 or 72 h. i, k Protein expression of TRIM24, STAT1, pSTAT1 and GAPDH, determined by western blot, in i CAL27 and k UMSCC22A cells accompanied by the protein band intensities relative to GAPDH in scramble and Trim24 siRNA-treated cells after 48 and 72 h (n = 3 samples per group). Data are presented as mean +/− SEM. #p value <0.1, *p value <0.05; **p value <0.01; ***p value <0.001 between groups.