Fig 1: T cell infiltration into tumors occurs independent of the gut and tumor microbiome(A) Study design for (B)–(K).(B) 16S rRNA levels in tumor and stool from mice orthotopically injected with cold (69) tumor cells (n = 10) or hot (2838c3) tumor cells and treated with (n = 15) and without (n = 20) antibiotics.(C and D) Quantification of CD3+ (C) and CD8+ and FOXP3+ (D) T cells from hot tumors of mice treated with (n = 19) or without (n = 13) antibiotics.(E) 16S rRNA levels in tumor from mice orthotopically injected with cold (69) tumor cells and treated with (n = 5) and without (n = 5) antibiotics.(F) Quantification of CD8+ and FOXP3+ T cells from cold tumors of mice treated with (n = 5) and without (n = 5) antibiotics.(G) Number of DEGs.(H) Bar graph displaying overrepresentation analysis of DEGs in indicated gene sets.(I and J) Tumor weights at day 20.(K) Quantification of intra-tumoral CD19+ cells.(L and M) Mean fluorescence intensity (MFI) of MHC class II (L) and CD206 (M) on CD11b+ F4/80+ intra-tumoral macrophages.Data were pooled from two to three experiments (B–D and J–M) or are representative of two independent experiments (E–I). Statistical significance was calculated using one-way ANOVA with Dunnett’s test (B, L, and M) and a two-tailed Mann-Whitney test (C–F and I–K). Data are represented as scatterplots (mean ± SD). NS, not significant.