Fig 1: FLASH regulates the expression of genes with roles in cell junction formation and EMT. (A-C) Gene expression profile in Mock, FLASH, NPAT, SLBP, and ZEB1-depleted cells was evaluated by qPCR. (A) Graphs show mRNA levels of FLASH, NPAT, SLBP, and ZEB1. (B-C) Four representative upregulated genes, CDH1, CGN, CADM3, and MARVELD3 (B), and four representative downregulated genes, EMB, LYRIC, RUFY3, and PJA2 (C) are shown. The significance of differences was confirmed by Student t-test for silencing efficiency (*, p < 0.05; **, p < 0.01). (D) Western blot analysis of genes tested by qPCR in A-C. Actin was used as the loading control.