Fig 1: Anti-PD-1 therapy or naive CD8+ T-cells combination with anti-PD-1 therapy increases the levels of perforin, FasL and GrB, but decreases PI-9 level, while treatment with NSP reverses the expression of these factors. (A) The serum concentrations of perforin and FasL were measured by ELISA. (B) The mRNA expression of perforin, GrB, PI-9 and FasL was detected by qRT-PCR. (C) The protein levels of perforin, GrB, PI-9 and FasL were determined by western blotting.*p < 0.05; **p < 0.01; ***p < 0.001.FasL: Fas-ligand; GrB: Granzyme B; NSP: Neuroserpin; PD-1: Programmed death 1; PI-9: Proteinase inhibitor 9.