Fig 1: Mlkl deficiency does not alter differentiation into beige adipocytesData were obtained in 3T3-L1 cells with a CRISPR/Cas-9-mediated Mlkl-KO and 3T3-L1 cells transfected with a Cas9/scramble gRNA plasmid corresponding to CTL cells. p values were determined by ANOVA with the Geisser-Greenhouse correction and Tukey’s multiple comparisons test. Results are expressed as means ± SEM.(A) Representative images of fluorescence microscopy after staining of Oil Red O (Red) or Perilipin-1 (Green) and nuclei (Blue). Images are representative of three independent experiments of differentiated CTL and Mlkl-KO cells at D10.(B) Triglyceride content measured in CTL and Mlkl-KO cells at 10 days post-differentiation (White vs. beige adipocytes). # indicates that triglyceride levels in white adipocyte-oriented Mlkl-KO cells are significantly decreased compared to all other groups.(C) Immunofluorescence quantification of Perilipin-1. # indicates that immunofluorescence level of Perilipin-1 is significantly decreased compared to all other groups.(D) Representative images of fluorescence microscopy after staining Ucp-1 (Green) and nuclei (Blue). Images are representative of three independent experiments of differentiated CTL and Mlkl-KO cells at D10.(E) Immunofluorescence quantification of UCP-1. # indicates that immunofluorescence level of UCP-1 is significantly decreased compared to the groups of beige adipocytes.(F) Ucp-1 mRNA expression levels in white and beige adipocytes at D10. **p < 0.001, *p < 0.05.(G) Ppar?, Pgc-1a, and Pgc-1ß mRNA expression levels in white and beige adipocytes at D3. *p < 0.05. See also Figures S3 and S7.