Fig 2: EVDC@Mn‐DNA is uptaken by dendritic cells through membrane fusion. A) Representative images of mouse DCs after treated with Dil (red) labeled EVDC encapsulating Mn‐FAM (green) labeled DNA over time. DAPI stained nuclei. B) FACS analysis of mouse DCs after treated with (Dil)EVDC@Mn‐(FAM)DNA over time. Bar chart shows the relative mean Dil fluorescence intensity in each group over time (n = 3 independent experiments). C) Representative images of mouse DCs after treated with FAM labeled Mn‐DNA complex, (Dil)EVDC@Mn‐DNA or (Dil)EVDC@Mn‐(FAM)DNA for 6 h are given (n = 3 independent experiments). White boxes indicate the magnified areas. D,E) Distribution graph illustrates the distance pixel between Dil channel (red), FAM channel (green) and DAPI‐stained nucleus (blue) in mouse DCs following treatment with (Dil)EVDC@Mn‐DNA or (Dil)EVDC@Mn‐(FAM)DNA. Gray value represents the intensity of each fluorescence signal. F) Representative confocal images of DiO or FITC conjugated anti‐LAMP1 stained DCs after treated with (Dil)EVDC@Mn‐DNA are given (n = 3 independent experiments). White boxes indicate the magnified areas. G,H) Distribution graph illustrates the distance pixel between Dil channel (red), DiO channel/FITC conjugated anti‐LAMP1 stained lysosome (green) and DAPI‐stained nucleus (blue) in (Dil)EVDC@Mn‐DNA treated mouse DCs. Gray value represents the intensity of each fluorescence signal. I) Representative fluorescent images of cGAS, Z‐DNA, and DAPI staining in DCs following the indicated treatments are provided. Magnified views are displayed on the right‐hand side, with white arrows highlighting cGAS phase separation. Results represent mean ± S.E.M. B) One‐way ANOVA with Tukey's post hoc test. Scale bars in (A, C, F) represent 50 µm. I) 5 µm.