SARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit mAb 99423S from Cell Signaling Technology

Product Specs
ItemSARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit mAb
CompanyCell Signaling Technology
Price $357.00 Inquire
Catalog Number99423S
ClonalityMonoclonal
ReactivityVir
ApplicationsW, IHC-P, IF-IC
HostRabbit
Quantity100 µl
TargetSpike Protein SARS-CoV-2
NCBI Gene Aliasesspike glycoprotein

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) SARS-CoV-2 Omicron BA.2.86 and JN.1 expand tropism in human proximal intestinal epitheliumNature CommunicationsJune 5, 2026Kenrie PY Hui, John CW Ho, Ka-Chun Ng, Jenny CM Chan, Taylor WC Ho, Rachel HH Ching, Leo LM Poon, Malik Peiris, John M Nicholls, Michael CW Channormal goat serum for 10 min at room temperature (RT) and then incubated with either SARS-CoV-2 spike (99423, Cell Signalling) or SARS-CoV-2 N (40143, Sino Biological) antibodies for 90 min at RT followed by alkaline

(2) SARS-CoV-2 spike protein exerts an anti-cancer effect in A549 cells in association with MEG3 and BCYRN1 regulationScientific ReportsMay 4, 2026Jung-Eun Kim, Hengmin Han, Leeseon An, Eunseo Park, Hyo-Jeong Leeat room temperature for 5 min followed by overnight incubation at 4 °C with Spike 1 antibody (Cat: 99423, Cell Signaling, Danvers, MA, USA) in 1% BSA in PBST. After washing, cells were incubated for 1 h in

(3) Extracellular Vesicle‐Delivered tRF‐His‐GTG‐1 Reprograms Neutrophil Lipophagy and Triggers Inflammation in COVID‐19Advanced ScienceJanuary 14, 2026Tsai‐Ling Liao, Po‐Yu Liu, Yi‐Ming Chen, Kuo‐Tung Tang, Hung‐Jen Liu, Der‐Yuan Chentissue sections were incubated with either anti–SARS‐CoV‐2 spike antibody (Cell Signaling Technology, #99423) or antineutrophil elastase (NE) antibody (Santa Cruz Biotechnology, sc‐53388). Ten randomly selected

(4) Effectiveness of a broad-spectrum bivalent mRNA vaccine against SARS-CoV-2 variants in preclinical studiesEmerging microbes & infectionsDecember 1, 2024Lu J, Tan S, Gu H, Liu K, Huang W, Yu Z, Lu G, Wu Z, Gao X, Zhao J, et al.#42172 (42172S, Cell Signaling, MA, USA) SARS-CoV-2 Spike Protein (S1) (E5S3 V) Rabbit mAb #99423 (99423S, Cell Signaling), SARS-CoV-2 (2019-nCoV) Spike S2 Antibody (40590-D001, Sino Biological, Beijing, China),

(5) The Novel A-Type Proanthocyanidin-Rich Phytocomplex SP4™ Acts as a Broad-Spectrum Antiviral Agent against Human Respiratory VirusesInternational journal of molecular sciencesJuly 5, 2024Sibille G, Mannino G, Frasson I, Pavan M, Luganini A, Salata C, Maffei ME, Gribaudo G.NY, USA) (diluted 1:500) or the anti-SARS-CoV-2 spike protein (S1) rabbit monoclonal antibody (mAb) (99423; Cell Signaling Technologies; Danvers, MA, USA) (diluted 1:1000). Immunocomplexes were then detected

(6) SARS-CoV-2 Accessory Protein ORF8 Targets the Dimeric IgA Receptor pIgRVirusesJune 22, 2024Frederique Laprise, Ariana Arduini, Mathew Duguay, Qinghua Pan, Chen Liangcat. SC23948) or rabbit anti-SARS-CoV-2 spike protein (1:1000; Cell Signaling, Danvers, MA, USA; cat. 99423S) diluted in 2% bovine serum albumin (BSA; BioShop; cat. 9048-46-8) for 2 h at room temperature or with

(7) Corneal Stroma Analysis and Related Ocular Manifestations in Recovered COVID-19 PatientsInvestigative Ophthalmology & Visual ScienceMay 7, 2024Yuke Huang, Taiwei Chen, Xi Chen, Linxi Wan, Xiangtao Hou, Jiejie Zhuang, Jingyi Jiang, Yan Li, Jin Qiu, Keming Yu, et al.(CD147),Mouse,Santa Cruz (sc-21746),1:300 NRP1,Mouse,Santa Cruz (sc-5307),1:100 Spike,Rabbit,CST (99423S),1:100 IL-6,Rabbit,Proteintech (21865-1-AP),1:200 NFkB-p65,Rabbit,CST (8242T),1:200 CD34,Mouse,Proteintech

(8) Thapsigargin and Tunicamycin Block SARS-CoV-2 Entry into Host Cells via Differential Modulation of Unfolded Protein Response (UPR), AKT Signaling, and ApoptosisCellsApril 30, 2024Abeer Al Otaibi, Sindiyan Al Shaikh Mubarak, Fatimah Al Hejji, Abdulrahman Almasaud, Haya Al Jami, Jahangir Iqbal, Ali Al Qarni, Naif Khalaf Al Harbi, Ahmed Bakillahovernight at 4 °C with SARS-CoV-2 Spike Protein (S1) rabbit monoclonal antibody (Cell signaling, catalog #99423; 1:250 dilution). After three washes with PBS, spike proteins were detected with Alexa Fluor 488 goat

(9) Biodistribution of mRNA COVID-19 vaccines in human breast milkEBioMedicineOctober 1, 2023Hanna N, De Mejia CM, Heffes-Doon A, Lin X, Botros B, Gurzenda E, Clauss-Pascarelli C, Nayak A.Thermo Scientific, Rockford, IL, USA). The S protein expression was assayed by anti-S antibody (cat# 99423, Cell Signaling Technology, Danvers, MA, USA)27 detection using an automated capillary western blot

(10) Pulmonary fat embolism: a potentially new fatal complication of SARS-CoV-2 infection. A case reportBMC Infectious DiseasesSeptember 4, 2023Jiangwei Kong, Tengfei Yang, Fu Zhang, XinBiao Liao, Sihao Du, Xingan Yang, Dongri Lithe PFE (Fig. 1b) and microthrombi (Fig. 1c-d). Immunohistochemistry of the spike protein (Cat. No. 99423, Cell Signaling Technology, Danvers, MA) and nucleocapsid protein (Cat. No. 26369, Cell Signaling Technology,

(11) SARS-CoV-2 protein ORF8 limits expression levels of Spike antigen and facilitates immune evasion of infected host cellsThe Journal of biological chemistryAugust 1, 2023Kim IJ, Lee YH, Khalid MM, Chen IP, Zhang Y, Ott M, Verdin E.anti-anti-Spike S2, Cell Signaling, Cat #: 27620, 1:2,000 dilution) (rabbit anti-Spike S1, Cell Signaling, Cat #: 99423, 1:2,000 dilution) (mouse anti-Strep, Qiagen, Cat #: 34850, 1:2,000 dilution) (rabbit anti-ORF8, GeneTex,

(12) Neutralizing and Enhancing Epitopes of the SARS-CoV-2 Receptor-Binding Domain (RBD) Identified by NanobodiesVirusesMay 26, 2023Kanasap Kaewchim, Kittirat Glab-ampai, Kodchakorn Mahasongkram, Thanatsaran Saenlom, Watayagorn Thepsawat, Monrat Chulanetra, Kiattawee Choowongkomon, Nitat Sookrung, Wanpen Chaicumpaanti-6× His antibody (Invitrogen) and rabbit anti-S1 subunit of SARS-CoV-2 spike protein (S1 mAb, Cat. 99423S; Cell signaling, Danvers, MA, USA) at dilutions of 1:200 and 1:300, respectively, for 1 h and washed.

(13) Reduced Pathogenicity and Transmission Potential of Omicron BA.1 and BA.2 Sublineages Compared with the Early Severe Acute Respiratory Syndrome Coronavirus 2 D614G Variant in Syrian HamstersThe Journal of Infectious DiseasesMay 12, 2023Wen Su, Ka Tim Choy, Haogao Gu, Sin Fun Sia, Ka Man Cheng, Sarea Islam Nuha Nizami, Pavithra Krishnan, Yuet Mai Ng, Lydia Dai Jia Chang, Yingzhi Liu, et al.anti-N monoclonal antibody (no. 40143-MM05; Sinobiological), or rabbit anti-S1 monoclonal antibody (no. 99423; Cell Signaling) and counterstained with hematoxylin. Images were captured using a Nanozoomer slide

(14) Liver alterations and detection of SARS-CoV-2 RNA and proteins in COVID-19 autopsiesGeroScienceApril 1, 2023Pesti A, Danics K, Glasz T, Várkonyi T, Barbai T, Reszegi A, Kovalszky I, Vályi-Nagy I, Dobi D, Lotz G, et al.and to detect SARS-CoV-2 spike (S1) protein a rabbit monoclonal antibody (IgG1, clone E5S3V, Cat. No. 99423, Cell Signaling Technology, Danvers, MA) in 1:500 dilution was used. The sensitivity and specificity

(15) Lipid Raft Integrity and Cellular Cholesterol Homeostasis Are Critical for SARS-CoV-2 Entry into CellsNutrientsAugust 19, 2022Ahmed Bakillah, Fatimah Al Hejji, Abdulrahman Almasaud, Haya Al Jami, Abbas Hawwari, Ali Al Qarni, Jahangir Iqbal, Naif Khalaf Alharbiprimary antibodies overnight at 4 °C (anti-spike protein monoclonal antibody; Cell signaling, catalog #99423; 1:500 dilution). After three washes with PBS, spike proteins were detected with Alexa Fluor 488 goat

(16) Effect of apigetrin in pseudo-SARS-CoV-2-induced inflammatory and pulmonary fibrosis in vitro modelScientific reportsJanuary 1, 0001"Han H, Kim JE, Lee HJ."at − 20 °C) at room temperature for 5 min. The fixed cells were exposed to the diluted Spike 1 (Cat: 99423, Cell Signaling, Danvers, MA, USA) antibody in 1% BSA in PBST in a humidified chamber overnight at 4

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SARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit mAb from Cell Signaling Technology

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SARS-CoV-2 Spike Protein (S1) (E5S3V) Rabbit mAb from Cell Signaling Technology

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