Fig 1: Determination of the P.g. effects on OSCC cells via IL-6. (A) Concentration of IL-6 in the oral lavage fluid samples collected from different clinical patients. The independent experiment was performed twice and the error bars were presented as SEM result. (B) IL-6R expression feature of the primary OSCC cells with or without the treatment of IL-6. The SCCA determination was displayed as Y axis for confirming the primary OSCC cells, the IL-6R determination was displayed as X axis. (C) Cell proliferation assay of IL-6R negative (IL-6R-) and IL-6R positive (IL-6R+) primary OSCC cells when treated or not with IL-6. (D) Cell migration assay of IL-6R negative (IL-6R-) and IL-6R positive (IL-6R+) primary OSCC cells when treated or not with IL-6. (E) Quantification result of the cell migration assay. Each experiment with cells was performed with triplication of samples (N = 3) and the error bars were presented as SD result. The independent experiment was performed at least three times. For the statistic test using, results in panel A, C and E were analyzed with One-way ANOVA method.