Fig 2: HOXA10 is a downstream target of miR-588. (a) Starbase was used for target prediction between HOAX10 and miR-388. (b-e) The combination between HOXA10 and miR-588 was conducted through the dual-luciferase reporter (b-c) and RIP assays (d-e). (f) HOXA10 mRNA expression was examined via RT-qPCR. (g) The relationship between HOXA10 and miR-588 expression was analyzed by Pearson’s correlation. (h-i) The protein level of HOXA10 was measured via western blotting. **P < 0.01, ***P < 0.001, ****P < 0.0001.

Fig 3: MiR-588 acts as a tumor inhibitor and radiation sensitizer by targeting HOXA10. The transfection of miR-NC, miR-588, miR-588+pcDNA, and miR-588+HOXA10 was performed in A549 and H1299 cells. (a) Western blotting was used for the analysis of HOXA10 protein expression. (b-c) The CCK-8 and EdU assays were used to determine cell viability (b) and proliferation (c), respectively. (d) Flow cytometry was used for apoptosis examination. (e-f) The transwell and wound healing assays were applied for the assessment of invasion (e) and migration (f), respectively. (g-h) Western blotting was applied for the detection of c-myc and cleaved-caspase-3. (i-j) The colony formation assay was used for survival analysis after cells were treated with radiation. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Fig 4: Graphical abstract of circ_0010235/miR-588/HOXA10 axis in NSCLC progression and radioresistance.

Fig 5: Melatonin administration improved endometrial receptivity during the implantation state. (A) Effects of melatonin on mRNA levels of endometrial receptivity markers (LIF, integrin β3, HoxA10, and HoxA11) by qRT-PCR in endometrium during the window of implantation between the control, adenomyosis model, and melatonin treated mice. (B) Western blot gels of LIF, integrin β3, HoxA10, and HoxA11. (C) Bar graph showing the quantification of LIF/GAPDH, integrin β3/GAPDH, HoxA10/GAPDH, and HoxA11/GAPDH, respectively. (D) Images of immunohistochemistry for LIF, integrin β3, HoxA10, and HoxA11, and (E) corresponding quantified expression values. ****P<0.0001; *** P<0.001; **P<0.01; *P<0.05; n=6. mRNA, messenger RNA; LIF, leukemia inhibitory factor; HoxA10, homeobox A10; HoxA11, homeobox A11; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; qRT-PCR, quantitative reverse transcription polymerase chain reaction.