Fig 2: PKMYT1 knockdown inhibits the migration, invasion and EMT of SCC-9 cells by regulating CCNA2 expression. (A) Relative migration rate was detected by using a wound healing assay. (B) Relative cell invasion rate was detected by using a Transwell assay. (C) MMP2 and MMP9 expression was detected by using western blot analysis. (D) EMT-related protein expression was detected by using western blot analysis. **P<0.01, ***P<0.001. PKMYT1, protein kinase, membrane-associated tyrosine/threonine 1; EMT, epithelial-mesenchymal transition; CCNA2, cyclin A2; sh, short hairpin; ZEB1, zinc finger E-box binding homeobox 1.

Fig 3: Cell cycle regulator proteins expression in different CTR groups. A–H the expression of CCNA2, CCNB1, CCND1, TP53, P16, P21, TOP2A, and pRb in lung adenocarcinoma with different CTR. CTR, consolidation-to-tumour ratio

Fig 4: Associations between Ki-67 and cell cycle regulator proteins expression. A–D the expression of the following four cell cycle regulator proteins showed a positive correlation with Ki-67 expression, namely CCNA2, TOP2A, TP53, and pRb, E–H the expression of the remaining four cell cycle regulator proteins (included CCNB1, CCND1, P16 and P21) is not correlated with Ki-67 expression

Fig 5: Differences in the positive expression rate of cell cycle regulatory proteins among different CTR groups. A the TMA constructed using clinical samples (HE staining); B the positive expression rates of GGO-LUAD and SN-LUAD on eight cell cycle regulatory proteins (CCNA2, CCNB1, CCND1, P16, P21, TOP2A, TP53, and pRb). CTR consolidation-to-tumour ratio, TMA tissue microarrays