Fig 1: Characterize the cuprizone induced demyelination of central nervous system in mice.Schematic illustration of the experimental protocols, the mice fed with normal diet and 0.2% cuprizone administration diet in control group and cpz-induced group for 8 weeks, respectively (A). The body weight of the mice was measured weekly according to the experimental requirement (B). IF staining of MBP, MAG, MOG and GFAP in the corpus callosum of normal fed mice compared with cuprizone fed group (C). Luxol fast blue (LFB) staining of the CPZ-fed and control groups in the corpus callosum region (D). Western blot analysis of the protein expression of MBP, MAG, MOG and GFAP in the control group and cpz-induced group of corpus callosum (E). Statistical analysis of MBP, MAG, MOG and GFAP protein expression level by grey value analysis (F). *p < 0.05, **p < 0.01, scale bar 50 μm.