Fig 1: Baf155 ablation does not affect the stability of components of SWI/SNF complex in the skeletal muscle.(A) RT-qPCR analysis of the expression of Baf155 in hind limb skeletal muscles (TA, EDL, GA, SOL, Q) of Baf155WT and Baf155ΔMF mice (n = 3 mice per genotype). (B, C) Representative immunoblotting analysis of Baf155 (B) and the densitometric quantification of relative Baf155 protein level in the Q muscle of Baf155ΔMF mice compared to Baf155WT mice (n = 3 mice per genotype) (C). (D, E) Representative immunoblotting analysis of Brg1, Snf5, and Baf170 (D) and the densitometric quantification of relative level of each indicated protein in Q muscle of Baf155ΔMF mice compared to Baf155WT mice (n = 3 mice per genotype) (E). (F) RT-qPCR analysis of the expressions of Brg1, Snf5, and Baf170 in Q muscle of Baf155WT and Baf155ΔMF mice (n = 3 mice per genotype). (G, H) Representative immunoblotting analysis of Arid1a, Brm, Baf60a, Baf60b, and Baf60c (G) and the densitometric quantification of relative protein level of each indicated protein in Q muscle of Baf155ΔMF mice compared to Baf155WT mice (n = 3 mice per genotype) (H). Each lane in immunoblotting analysis (B, D, and G) indicates each mouse (biological replicate), and each dot in the graphs (A, C, E, F, and H) represents each mouse (biological replicate). Data are presented as mean ± SEM of biological replicates. Statistical analyses were performed using unpaired Student’s t test (n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001 versus Baf155WT control). The data underlying this figure can be found in S1 Data. Arid1a, AT-rich interaction domain 1a; Baf60, Brg1/Brm-associated factor 60; Baf155, Brg1/Brm-associated factor 155; Baf170, Brg1/Brm-associated factor 170; Brg1, Brahma-related gene 1; Brm, Brahma; EDL, extensor digitorum longus; GA, gastrocnemius; MF, myofiber; RT-qPCR, reverse transcription quantitative real-time PCR; SEM, standard error of the mean; Snf5, sucrose non-fermentable 5; SOL, soleus; TA, tibialis anterior; WT, wild type; Q, Quadriceps.