Fig 1: TAM activated TLR4 signaling and inactivated immune checkpoint SHP1/SHP2. (A) The protein expression levels of the TLR4/NF-?B signaling pathway normalized to the GAPDH in RAW 264.7 cells were detected. (B) The expression levels of SIRPa protein, the phosphorylation levels of SHP1 and SHP2 proteins in RAW 264.7 cells were detected using Western blot. (C,D) The expression levels of SIRPa protein, the phosphorylation levels of SHP1 and SHP2 proteins in GH3 and AtT-20 cells were detected. (E) The expression levels of SIRPa protein, the activity of SHP1 and SHP2 proteins in tumor tissues were also detected. Bars indicates SEM, VC vehicle control, refers to tumor-bearing mice treated with the vehicle. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control or VC. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. cells treated with IL-TAM: tamoxifen.