Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.