The EB3 Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to MAPRE3. This antibody recognizes Human, Mouse, and Rat antigen. The EB3 Antibody has been shown to work in the following applications: EIA, Immunoassay, and ELISA.
Description
Function: Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). May play a role in cell migration (By similarity).
Subcellular Location: Cytoplasm>Cytoskeleton. Note: Associated with the microtubule network. Detected at the plus end of microtubules.
Tissue Specificity: Predominantly expressed in brain and muscle.
Subunit Structure: Interacts with APC2 (PubMed:10644998). Homodimer (PubMed:19255245). Heterodimer with MAPRE1 (PubMed:19255245). Interacts with DCTN1 and SRCIN1 (PubMed:14514668, PubMed:19146815). Binds to the C-terminal domain of APC (PubMed:14514668). Binds monomeric and polymerized tubulin (PubMed:10188731). Interacts (via C-terminus) with CLIP1 (PubMed:17563362). Interacts with SLAIN2 and SLAIN1 (PubMed:21646404). Interacts with AKAP9 (PubMed:28814570). Interacts with PDE4DIP (PubMed:28814570). Interacts with PDE4DIP isoform 13/MMG8/SMYLE; this interaction is required for its recruitment to the Golgi apparatus (PubMed:25217626).
Similarity: Composed of two functionally independent domains. The N-terminal domain forms a hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.Belongs to the MAPRE family