The Rabbit Anti-GSK3 Antibody from MyBioSource.com is a Rabbit Monoclonal antibody to GSK3A. This antibody recognizes Human, Mouse, and Rat antigen. The Rabbit Anti-GSK3 Antibody has been shown to work in the following applications: Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blot.
Description
Glycogen synthase kinase-3alpha (GSK-3alpha) and GSK-3beta are highly similar isoforms of serine/ threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3beta activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFalpha in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3alpha and GSK-3beta induces phosphorylation of Ser 21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3alpha or Ser 9 of GSK-3beta and inactivates both forms. GSK-3alpha/beta is positively regulated by phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3alpha/beta participates in energy metabolism, neuronal cell development, and body pattern formation. Tyrosine dephosphorylation of GSK-3 is involved in its extracellular signal-dependent inactivation