MyBioSource.com's Rabbit Anti-Talin Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, Immunocytochemistry, and Western Blot. The Rabbit Anti-Talin Antibody was generated using Talin 1, and TLN1 as the antigen and it reacts with Human, Mouse, Rat, Chicken/Bird, and Zebrafish/Fish.
Description
Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240kDa) into an amino-terminal globular head domain of 47kDa and a carboxyl-terminal 190kDa rod domain. The talin head domain contains a FERM domain that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation, and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration