MyBioSource.com's Rabbit Anti-Histone H3, phosphorylated Antibody is a Rabbit monoclonal antibody. The Rabbit Anti-Histone H3, phosphorylated Antibody was generated using H3 clustered histone 1, H3 clustered histone 6, H3C1, and H3C6 as the antigen. It reacts with Human. This antibody has been shown to work in applications such as: Immunohistochemistry, and Immunohistochemistry - fixed.
Description
Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0. 0 to 6. 6% (median value 0. 8%). Increased expression of PHH3 was significantly associated with tumor thickness (p = 0. 031), presence of tumor ulceration (p =0. 041) and tumor necrosis (p =0. 027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0. 003) and high Ki-67 expression (p = 0. 002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis