MyBioSource.com's Anti-PCNA Purified is a Mouse monoclonal antibody. The Anti-PCNA Purified Antibody was generated using PCNA, and proliferating cell nuclear antigen as the antigen. It reacts with Human, Mouse, Rat, Chicken/Bird, Drosophila/Arthropod, and Non-Human Primate. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry - fixed, Immunohistochemistry - frozen, Immunoprecipitation, and Western Blot.
Description
PCNA (proliferating cell nuclear antigen), which is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents