The Rabbit Anti-Myosin IIA Heavy Chain Antibody from MyBioSource.com is a Rabbit Polyclonal antibody to MYH9. This antibody recognizes Human, Mouse, Rat, and Chicken/Bird antigen. The Rabbit Anti-Myosin IIA Heavy Chain Antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, and Western Blot.
Description
Non-muscle myosin II is an actin-based motor protein essential to cell motility, division, migration, adhesion and polarity. This myosin forms a hexameric complex comprised of two heavy chains (NMHC-II), two essential light chains, and two regulatory light chains (RLC). In vertebrates, there are threenMHC-II isoforms (NMHC-IIA, nMHC-IIB, andnMHC-IIC), which exhibit distinct patterns of expression in cells and tissues. Regulation ofnMHC-II activity occurs through RLC and HC phosphorylation. RLCs are phosphorylated at Thr-18 and Ser-19, which activates myosin II motor activity and promotes filament stability. By contrast, PKC phosphorylation of Ser-1/Ser-2 and Thr-9 in RLC may decrease activated myosin II interaction with actin. Several kinases phosphorylationnMHC-II isoforms directly. TRPM7 phosphorylates Thr-1800, Ser-1803, and Ser-1808, which reducesnMHC-IIA incorporation into the actin cytoskeleton. PKC phosphorylatesnMHC-IIA on Ser-1916 andnMHC-IIB on multiple tailpiece serines leading to inhibition of filament assembly. Casein kinase II phosphorylatesnMHC-IIA on Ser-1943 and increases disassembly ofnMHC-IIA filaments