Figure A. FITC conjugated Rat anti Mouse CD4 and Alexa Fluor 700 conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD4 and Alexa Fluor 700 conjugated Rat anti Mouse CD3 . All experiments performed on mouse blood gated on live single lymphocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer

Figure A. FITC conjugated Rat anti Mouse CD4 antibody, clone RM4-5 (MCA2691F) and Alexa Fluor^® 700 conjugated Rat IgG2a isotype control (MCA1212A700).
Figure B. FITC conjugated Rat anti Mouse CD4 antibody, clone RM4-5 (MCA2691F) and Alexa Fluor^® 700 conjugated Rat anti Mouse CD3 antibody, clone KT3 (MCA500A700). All experiments performed on mouse blood gated on live single lymphocytes, in the presence of 10% mouse serum.
Data acquired on the ZE5 Cell Analyzer.