Fig 1: Prosaposin immunoreactivity in the cochlear (CN) and vestibular nuclei (VN). (A) Prosaposin immunoreactivity with hematoxylin counterstaining in CN. Immunoreactivity was widely distributed in CN (circled by dashed line). (B) High-magnification squared in (A). The dot-like immunostaining pattern in the cell body was observed in the large- (arrows) and small-sized cells (arrowhead). (C) Neuronal marker PGP9.5 immunoreactivity in CN. Only neurons were stained by anti-PGP9.5 antibody. (D) Prosaposin immunoreactivity in CN. (E) Double immunofluorescence staining of PGP9.5 and prosaposin in CN. Prosaposin immunoreactivities were found in PGP9.5-positive (arrows) and PGP9.5-negative (arrowhead) cells. (F) Prosaposin immunoreactivity in VN near the lateral fourth ventricle (4V). Immunoreactivity was widely distributed in VN. (G) High magnification squared in (F). Prosaposin immunoreactivity in VN was similar to that in CN. Arrows indicate that the large cells contained dot-like prosaposin immunoreactivities in soma, and arrowhead indicates small-sized cell showing prosaposin immunoreactivity. (H) PGP9.5 immunoreactivity in VN. (I) Prosaposin immunoreactivity in VN. (J) Double immunofluorescence staining of PGP9.5 and prosaposin in VN. Prosaposin immunoreactivities were found in PGP9.5-positive (arrows) and PGP9.5-negative (arrowheads) cells. (K) Double immunofluorescence staining of GFAP and prosaposin in CN. Arrowhead indicates prosaposin immunoreactivity in GFAP-positive cell. (L) Double immunofluorescence staining of GFAP and prosaposin in VN. Arrowhead indicates prosaposin immunoreactivity in GFAP-positive cell.