Bioassay Technology Laboratory's LAT(Phospho Tyr255) Rabbit Polyclonal Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: EIA, Immunoassay, ELISA, and Western Blot.
Description
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling: both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores: PKC activation: MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1: GRB2: GRAP2: and other signaling molecules.:miscellaneous:Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC: maybe by inducing the dephosphorylation of LAT.:PTM:Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.:PTM:Phosphorylated on tyrosines by ZAP-70 upon TCR activation: or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement.:subcellular location:Present in lipid rafts.:subunit:When phosphorylated: interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2: GRAP: GRAP2: PLCG1 and PLCG2. Interacts indirectly with CBL: SOS: VAV: and LCP2. Interacts with SHB: SKAP2 and CLNK (By similarity). Interacts with FCGR1A.:tissue specificity:Expressed in thymus: T-cells: NK cells: mast cells and: at lower levels: in spleen. Present in T-cells but not B-cells (at protein level).: