The Histone H4 (Tri Methyl Lys21) Rabbit Polyclonal Antibody from Bioassay Technology Laboratory is a Rabbit Polyclonal antibody. This antibody recognizes Human, Mouse, and Rat antigen. The Histone H4 (Tri Methyl Lys21) Rabbit Polyclonal Antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, and Western Blot.
Description
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin: limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation: DNA repair: DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones: also called histone code: and nucleosome remodeling.:PTM:Acetylation at Lys-6: Lys-9: Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.:PTM:Citrullination at Arg-4 by PADI4 impairs methylation.:PTM:Monomethylated: dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.:PTM:Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.:PTM:Sumoylated: which is associated with transcriptional repression.:PTM:Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.:Belongs to the histone H4 family.:subunit:The nucleosome is a histone octamer containing two molecules each of H2A: H2B: H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.: