Immunohistochemistry (Paraffin) analysis of FAS (CD95) in paraffin-embedded human tonsil tissue using FAS Monoclonal Antibody (Product # MA5-38329) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot analysis of FAS (CD95) using FAS Monoclonal Antibody (Product # MA5-38329) at 1:2,000. Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, HL60 whole cell lysate, U87 whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 38, 12, 10, 17, 15, 36, 25 kDa. Observed band size: 45 kDa