Immunocytochemistry/Immunofluorescence analysis of eIF2b epsilon in NIH-3T3 cells using eIF2b epsilon Recombinant Monoclonal Antibody (Product # MA5-38175) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of eIF2b epsilon in extracts of various cell lines. Samples were incubated with eIF2b epsilon Monoclonal antibody (Product # MA5-38175) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.