Immunohistochemistry (Paraffin) analysis of GLAST in paraffin-embedded mouse brain tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with GLAST Recombinant Monoclonal Antibody (Product # MA5-41231) at a dilution of 1:50 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.